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https://hdl.handle.net/2440/81327
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Type: | Journal article |
Title: | X-ray fluorescence imaging of single human cancer cells reveals that the N-heterocyclic ligands of iodinated analogues of ruthenium anticancer drugs remain coordinated after cellular uptake |
Author: | Antony, S. Aitken, J. Vogt, S. Lai, B. Brown, T. Spiccia, L. Harris, H. |
Citation: | Journal of Biological Inorganic Chemistry, 2013; 18(7):845-853 |
Publisher: | Springer-Verlag |
Issue Date: | 2013 |
ISSN: | 0949-8257 1432-1327 |
Statement of Responsibility: | Sumy Antony, Jade B. Aitken, Stefan Vogt, Barry Lai, Tracey Brown, Leone Spiccia, Hugh H. Harris |
Abstract: | Analogues of KP1019 containing iodinated indazole ligands were prepared to investigate the biological fate of the Ru-N-heterocycle bond in this class of anticancer agents. The new complexes, 5-iodoindazolium trans-tetrachloridobis(5-iodoindazole)ruthen(III)ate (1) and 5-iodoindazolium trans-tetrachlorido(dimethyl sulfoxide)(5-iodoindazole)ruthen(III)ate (3), were characterized by elemental analysis, mass spectrometry and UV-vis spectrophotometry. Tetramethylammonium salts of these complexes (2 and 4) were synthesized and characterized in a similar manner. Half-maximum inhibitory concentrations of 2 and 4 with regard to A549 cells at 24 h were determined on the basis of the dose-response curves derived from real-time cell adhesion impedance measurements and were shown to be in the same range as those determined for KP1019 and NAMI-A using the same method. X-ray fluorescence imaging of single cultured A549 cells treated with 2 or 4 showed that, in both cases, the distribution of ruthenium and iodine was identical, indicating that the Ru-N bonds in the anionic complexes remained intact after incubation in culture medium and subsequent cellular uptake and processing. |
Keywords: | Anticancer drugs Ruthenium Iodine X-ray fluorescence imaging |
Rights: | © SBIC 2013 |
DOI: | 10.1007/s00775-013-1027-z |
Grant ID: | http://purl.org/au-research/grants/arc/DP0985807 http://purl.org/au-research/grants/arc/DP0984722 |
Published version: | http://dx.doi.org/10.1007/s00775-013-1027-z |
Appears in Collections: | Aurora harvest Chemistry and Physics publications |
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