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|Title:||Cloning and analysis of the human complement factor H gene promoter|
|Citation:||Immunology and Cell Biology, 1997; 75(5):508-510|
|Publisher:||Nature Publishing Group|
|Helena M Ward, Naomi H Higgs, Timothy K Blackmore, Tania A Sadlon and David L Gordon|
|Abstract:||The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements, cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.|
|Keywords:||Cloning; Complement; Gene promoter|
|Rights:||Copyright status unknown|
|Appears in Collections:||Medical Education Unit publications|
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