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Type: Journal article
Title: Cloning and analysis of the human complement factor H gene promoter
Author: Ward, H.
Higgs, N.
Blackmore, T.
Sadlon, T.
Citation: Immunology and Cell Biology, 1997; 75(5):508-510
Publisher: Nature Publishing Group
Issue Date: 1997
ISSN: 0818-9641
Statement of
Helena M Ward, Naomi H Higgs, Timothy K Blackmore, Tania A Sadlon and David L Gordon
Abstract: The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements, cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.
Keywords: Cloning
Gene promoter
Rights: Copyright status unknown
DOI: 10.1038/icb.1997.79
Appears in Collections:Aurora harvest 4
Medical Education Unit publications

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