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https://hdl.handle.net/2440/82216
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dc.contributor.author | Fauser, J. | - |
dc.contributor.author | Matthews, G. | - |
dc.contributor.author | Cummins, A. | - |
dc.contributor.author | Howarth, G. | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Chemotherapy: international journal of experimental and clinical chemotherapy, 2014; 59(3):214-224 | - |
dc.identifier.issn | 0009-3157 | - |
dc.identifier.issn | 1421-9794 | - |
dc.identifier.uri | http://hdl.handle.net/2440/82216 | - |
dc.description.abstract | <h4>Background</h4>Fatty acids are classified as short-chain (SCFA), medium-chain (MCFA) or long-chain and hold promise as adjunctive chemotherapeutic agents for the treatment of colorectal cancer. The antineoplastic potential of MCFA remains underexplored; accordingly, we compared the MCFA lauric acid (C12:0) to the SCFA butyrate (C4:0) in terms of their capacity to induce apoptosis, modify glutathione (GSH) levels, generate reactive oxygen species (ROS), and modify phases of the cell cycle in Caco-2 and IEC-6 intestinal cell lines.<h4>Methods</h4>Caco-2 and IEC-6 cells were treated with lauric acid, butyrate, or vehicle controls. Apoptosis, ROS, and cell cycle analysis were determined by flow cytometry. GSH availability was assessed by enzymology.<h4>Results</h4>Lauric acid induced apoptosis in Caco-2 (p < 0.05) and IEC-6 cells (p < 0.05) compared to butyrate. In Caco-2 cells, lauric acid reduced GSH availability and generated ROS compared to butyrate (p < 0.05). Lauric acid reduced Caco-2 and IEC-6 cells in G0/G1and arrested cells in the S and G2/M phases. Lauric acid induced apoptosis in IEC-6 cells compared to butyrate (p < 0.05). Butyrate protected IEC-6 cells from ROS-induced damage, whereas lauric acid induced high levels of ROS compared to butyrate.<h4>Conclusion</h4>Compared to butyrate, lauric acid displayed preferential antineoplastic properties, including induction of apoptosis in a CRC cell line. | - |
dc.description.statementofresponsibility | J.K. Fauser, G.M. Matthews, A.G. Cummins, G.S. Howarth | - |
dc.language.iso | en | - |
dc.publisher | Karger | - |
dc.rights | © 2013 S. Karger AG, Basel | - |
dc.source.uri | http://dx.doi.org/10.1159/000356067 | - |
dc.subject | Medium-chain fatty acid | - |
dc.subject | Short-chain fatty acid | - |
dc.subject | Colorectal cancer | - |
dc.subject | Caco-2 | - |
dc.subject | IEC-6 | - |
dc.subject | Apoptosis | - |
dc.subject | Redox | - |
dc.subject | Cell cycle | - |
dc.subject | Glutathione | - |
dc.subject | Reactive oxygen species | - |
dc.title | Induction of apoptosis by the medium-chain length fatty acid lauric acid in colon cancer cells due to induction of oxidative stress | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1159/000356067 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Cummins, A. [0000-0003-3115-9498] | - |
dc.identifier.orcid | Howarth, G. [0000-0001-6979-6084] | - |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 4 |
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