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Type: Journal article
Title: Effect of granulocyte-macrophage colony-stimulating factor deficiency on ovarian follicular cell function
Author: Gilchrist, R.
Rowe, D.
Ritter, L.
Robertson, S.
Norman, R.
Armstrong, D.
Citation: Journal of Reproduction and Fertility, 2000; 120(2):283-292
Publisher: Society for Reproduction and Fertility
Issue Date: 2000
ISSN: 0022-4251
Abstract: Granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine secreted by lymphohaemopoietic and other cell lineages, is known to influence ovarian cyclicity and embryo development. The aim of this study was to examine the effect of GM-CSF on ovarian follicular cell function using GM-CSF-deficient (GM -/-) mice. Immature GM -/- and GM +/+ mice were stimulated with eCG, and cumulus-oocyte complexes and mural granulosa cells were collected 48 h later. Expression of GM-CSF receptor (GM-CSFR) alpha and beta mRNA subunits by cumulus-oocyte complexes and mural granulosa cells was examined using RT-PCR. Cumulus-oocyte complexes from both genotypes were found to express mRNA for the GM-CSFRalpha-subunit only, while the mural granulosa cells expressed both the alpha and beta receptor subunits. Cumulus-oocyte complexes recovered from GM -/- mice had approximately twice the number of cumulus cells per cumulus-oocyte complex than did those of GM +/+ mice (P < 0.05), even though the growth-promoting activity of denuded GM -/- oocytes was found to be equivalent to that of wild-type oocytes. GM-CSF deficiency was associated with marginally increased DNA synthesis in cumulus cells and significantly (P < 0.05) lower progesterone production by mural granulosa cells recovered from GM -/- compared with those recovered from GM +/+ mice. The addition of rec-mGM-CSF in vitro did not affect DNA synthesis in either cell type or progesterone production by mural granulosa cells, irrespective of GM-CSF status. There was no effect of GM-CSF deficiency on the capacity of FSH and insulin-like growth factor I to stimulate DNA synthesis in cumulus-oocyte complexes (approximately 15- and threefold, respectively) and in mural granulosa cells (approximately two- and threefold, respectively). Taken together, these data show that GM-CSF influences events associated with follicular maturation in mice. The effects of GM-CSF are not exerted directly in granulosa or cumulus cells, but appear to be mediated indirectly, perhaps through the agency of steroidogenesis-regulating secretions of local macrophage populations residing in the theca.
Keywords: Ovarian Follicle; Granulosa Cells; Cells, Cultured; Animals; Mice, Knockout; Mice; Progesterone; Follicle Stimulating Hormone; Insulin-Like Growth Factor I; Granulocyte-Macrophage Colony-Stimulating Factor; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; DNA; Analysis of Variance; Reverse Transcriptase Polymerase Chain Reaction; Dose-Response Relationship, Drug; Female
RMID: 0001000345
DOI: 10.1530/jrf.0.1200283
Appears in Collections:Obstetrics and Gynaecology publications

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