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https://hdl.handle.net/2440/8329
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Type: | Journal article |
Title: | Effect of granulocyte-macrophage colony-stimulating factor deficiency on ovarian follicular cell function |
Author: | Gilchrist, R. Rowe, D. Ritter, L. Robertson, S. Norman, R. Armstrong, D. |
Citation: | Reproduction, 2000; 120(2):283-292 |
Publisher: | Society for Reproduction and Fertility |
Issue Date: | 2000 |
ISSN: | 0022-4251 1741-7899 |
Abstract: | Granulocyte-macrophage colony-stimulating factor (GM-CSF), a cytokine secreted by lymphohaemopoietic and other cell lineages, is known to influence ovarian cyclicity and embryo development. The aim of this study was to examine the effect of GM-CSF on ovarian follicular cell function using GM-CSF-deficient (GM -/-) mice. Immature GM -/- and GM +/+ mice were stimulated with eCG, and cumulus-oocyte complexes and mural granulosa cells were collected 48 h later. Expression of GM-CSF receptor (GM-CSFR) alpha and beta mRNA subunits by cumulus-oocyte complexes and mural granulosa cells was examined using RT-PCR. Cumulus-oocyte complexes from both genotypes were found to express mRNA for the GM-CSFRalpha-subunit only, while the mural granulosa cells expressed both the alpha and beta receptor subunits. Cumulus-oocyte complexes recovered from GM -/- mice had approximately twice the number of cumulus cells per cumulus-oocyte complex than did those of GM +/+ mice (P < 0.05), even though the growth-promoting activity of denuded GM -/- oocytes was found to be equivalent to that of wild-type oocytes. GM-CSF deficiency was associated with marginally increased DNA synthesis in cumulus cells and significantly (P < 0.05) lower progesterone production by mural granulosa cells recovered from GM -/- compared with those recovered from GM +/+ mice. The addition of rec-mGM-CSF in vitro did not affect DNA synthesis in either cell type or progesterone production by mural granulosa cells, irrespective of GM-CSF status. There was no effect of GM-CSF deficiency on the capacity of FSH and insulin-like growth factor I to stimulate DNA synthesis in cumulus-oocyte complexes (approximately 15- and threefold, respectively) and in mural granulosa cells (approximately two- and threefold, respectively). Taken together, these data show that GM-CSF influences events associated with follicular maturation in mice. The effects of GM-CSF are not exerted directly in granulosa or cumulus cells, but appear to be mediated indirectly, perhaps through the agency of steroidogenesis-regulating secretions of local macrophage populations residing in the theca. |
Keywords: | Ovarian Follicle Granulosa Cells Cells, Cultured Animals Mice, Knockout Mice Progesterone Follicle Stimulating Hormone Insulin-Like Growth Factor I Granulocyte-Macrophage Colony-Stimulating Factor Receptors, Granulocyte-Macrophage Colony-Stimulating Factor DNA Analysis of Variance Reverse Transcriptase Polymerase Chain Reaction Dose-Response Relationship, Drug Female |
DOI: | 10.1530/jrf.0.1200283 |
Published version: | http://dx.doi.org/10.1530/reprod/120.2.283 |
Appears in Collections: | Aurora harvest 4 Obstetrics and Gynaecology publications |
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