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https://hdl.handle.net/2440/83532
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Type: | Journal article |
Title: | Identification and profiling of novel a1A-adrenoceptor-CXC chemokine receptor 2 heteromer |
Author: | Mustafa, S. See, H. Seeber, R. Armstrong, S. White, C. Sabatino, V. Ayoub, M. Pfleger, K. |
Citation: | Journal of Biological Chemistry, 2012; 287(16):12952-12965 |
Publisher: | Amer Soc Biochemistry Molecular Biology Inc |
Issue Date: | 2012 |
ISSN: | 0021-9258 1083-351X |
Statement of Responsibility: | Sanam Mustafa, Heng B. See, Ruth M. Seeber, Stephen P. Armstrong, Carl W. White, Sabatino Ventura, Mohammed Akli Ayoub, and Kevin D. G. Pfleger |
Abstract: | We have provided the first evidence for specific heteromerization between the α1A-adrenoceptor (α1AAR) and CXC chemokine receptor 2 (CXCR2) in live cells. α1AAR and CXCR2 are both expressed in areas such as the stromal smooth muscle layer of the prostate. By utilizing the G protein-coupled receptor (GPCR) heteromer identification technology on the live cell-based bioluminescence resonance energy transfer (BRET) assay platform, our studies in human embryonic kidney 293 cells have identified norepinephrine-dependent β-arrestin recruitment that was in turn dependent upon co-expression of α1AAR with CXCR2. These findings have been supported by co-localization observed using confocal microscopy. This norepinephrine-dependent β-arrestin recruitment was inhibited not only by the α1AR antagonist Terazosin but also by the CXCR2-specific allosteric inverse agonist SB265610. Furthermore, Labetalol, which is marketed for hypertension as a nonselective β-adrenoceptor antagonist with α1AR antagonist properties, was identified as a heteromer-specific-biased agonist exhibiting partial agonism for inositol phosphate production but essentially full agonism for β-arrestin recruitment at the α1AAR-CXCR2 heteromer. Finally, bioluminescence resonance energy transfer studies with both receptors tagged suggest that α1AAR-CXCR2 heteromerization occurs constitutively and is not modulated by ligand. These findings support the concept of GPCR heteromer complexes exhibiting distinct pharmacology, thereby providing additional mechanisms through which GPCRs can potentially achieve their diverse biological functions. This has important implications for the use and future development of pharmaceuticals targeting these receptors. |
Keywords: | 7-Helix Receptor Adrenergic Receptor Allosteric Regulation Arrestin Chemokines G Protein-coupled Receptors (GPCR) Heteromer |
Rights: | © 2012 by The American Society for Biochemistry and Molecular Biology, Inc. |
DOI: | 10.1074/jbc.M111.322834 |
Published version: | http://dx.doi.org/10.1074/jbc.m111.322834 |
Appears in Collections: | Aurora harvest 4 Medical Sciences publications |
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