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|Title:||Granulocyte-macrophage colony-stimulating factor: presence in human follicular fluid, protein secretion and mRNA expression by ovarian cells|
|Citation:||Molecular Human Reproduction, 1996; 2(8):555-562|
|Publisher:||OXFORD UNIV PRESS|
|Melinda J. Jasper, Mats Brännström, Jan I. OIofsson, Ossie M. Petrucco, Helen Mason, Sarah A. Robertson and Robert J. Norman|
|Abstract:||In recent years it has become evident that a leukocyte-cytokine network contributes to the paracrine regulation of ovarian function. The objectives of this study were to examine the presence of a potent lympho-haemopoietic cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), in tissues and fluids from human ovaries. In a prospective study, follicular fluid and plasma were collected from naturally cycling women and women undergoing hyperstimulation for in-vitro fertilization (IVF). Granulosa-lutein cells were collected at the time of oocyte recovery for IVF and corpora lutea were collected at the time of hysterectomy for non-ovarian reasons. Culture supernatants from ovarian cell and tissue cultures were harvested on completion of a 48 h incubation. Immunoactive GM-CSF was measured by enzyme-linked immunosorbent assay, and was found to be present at statistically significantly higher levels in follicular fluid (8.9 +/- 0.7 pg/ml) and plasma (11.3 +/- 0.8 pg/ml) of women undergoing hyperstimulation compared to follicular fluid (5.3 +/- 0.3 pg/ml) and plasma (7.1 +/- 0.5 pg/ml) from naturally cycling women. Immunoactive GM-CSF was also detected in culture supernatants of granulosa-lutein cells (47.6 pg/10(5) cells), early luteal phase corpora lutea (0.52 pg/microgram DNA) and mid-luteal phase corpora lutea (0.98 pg/microgram DNA). Furthermore, transcripts for GM-CSF, and both the alpha and beta subunits of the GM-CSF receptor, were detected by reverse transcription polymerase chain reaction (RT-PCR) in granulosa-lutein cell culture preparations and corpora lutea collected during the early, mid- and late luteal phase of the menstrual cycle. These results show that GM-CSF is expressed and secreted by cells within the human ovary, and, together with the finding of expression of mRNA for GM-CSF receptor, suggest a role for GM-CSF in the local regulation of ovarian events.|
|Keywords:||Ovary; Corpus Luteum; Luteal Cells; Follicular Fluid; Granulosa Cells; Cells, Cultured; Humans; Granulocyte-Macrophage Colony-Stimulating Factor; Receptors, Granulocyte-Macrophage Colony-Stimulating Factor; RNA, Messenger; DNA Primers; Polymerase Chain Reaction; Gene Expression; Base Sequence; Protein Conformation; Adult; Female; In Vitro Techniques|
|Description:||© European Society of Human Reproduction and Embryology 1996|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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