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Type: Journal article
Title: Epidermal Growth Factor and its receptor gene expression and peptide localization in porcine ovarian follicles
Author: Singh, B.
Rutledge, J.
Armstrong, D.
Citation: Molecular Reproduction and Development, 1995; 40(4):391-399
Publisher: Wiley-Liss
Issue Date: 1995
ISSN: 1040-452X
Statement of
Singh, Balwant ; Rutledge, Jean M. ; Armstrong, David T.
Abstract: <jats:title>Abstract</jats:title><jats:p>The present study was undertaken to determine the expression of genes for epidermal growth factor (EGF) and its receptor (EGF‐R) in various components of medium‐sized porcine ovarian follicles by reverse transcription‐polymerase chain reaction (RT‐PCR), and to localize their peptides during folliculogenesis by immunocytochemistry. A strong band for EGF mRNA transcript was detected in the oocyte, whereas the signal in cumulus, granulosa, and theca cells was very weak but detectable. In contrast, a very strong EGF‐R mRNA signal was observed in cumulus, granulosa, and theca cells, whereas the signal in the oocyte was very weak. EGF peptide was localized in the oocyte, cumulus, and granulosa cells of all stages of follicle. In the oocyte, the intensity of immunostaining was more pronounced in primordial and primary follicles, compared to antral follicles. In large antral follicles, immuno‐staining was pronounced in granulosa cells, whereas theca cells showed little or no detectable staining for EGF. EGF staining was also observed in the cumulus and granulosa cells of follicles undergoing atresia. EGF‐R immunostaining was observed in the oocytes of primordial and primary follicles, and in cumulus, granulosa, and theca cells of all stages of follicle, including atretic follicles. In large antral follicles, the intensity of immunostaining was more pronounced in theca cells than in granulosa cells, and the oocyte showed little or no detectable staining. No immunostaining was observed when the primary antibody was replaced with preimmune serum (EGF), or preabsorbed with the control peptide (EGF‐R), confirming the specificity of the staining procedures. These results suggest a local follicular production of EGF and its receptor in the porcine ovary, and thus a role for EGF of follicular origin in the regulation of follicular development in autocrine/paracrine fashion. © 1995 Wiley‐Liss, Inc.</jats:p>
Rights: © 1995 Wiley‐Liss, Inc.
DOI: 10.1002/mrd.1080400402
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Appears in Collections:Aurora harvest
Obstetrics and Gynaecology publications

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