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|Title:||Effect of 2,4-dinitrophenol on the energy metabolism of cattle embryos produced by in vitro fertilization and culture|
|Citation:||Reproduction Fertility and Development, 2002; 14(6):339-343|
|Publisher:||C S I R O Publishing|
|D. Rieger, L. T. McGowan, S. F. Cox, P. A. Pugh and J. G. Thompson|
|Abstract:||In cattle embryos, the proportion of ATP produced by glycolysis increases following the major activation of the embryonic genome, and development to the blastocyst stage is improved in the presence of 10 microM 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation, from Day 5 to Day 7 of culture. In Experiment 1 of the present study, culture of cattle embryos in the presence of 10 microM DNP from Day 5 to Day 7 stimulated development to the blastocyst stage, but had no significant effects on oxygen, pyruvate or glucose uptake, or on lactate production. In Experiment 2, culture of cattle embryos in the presence of 10 microM DNP from Day 5 to Day 7, stimulated the metabolism of [2-14C]pyruvate (a measure of Krebs cycle activity) on all of Days 5, 6 and 7, and stimulated metabolism of [5-3H]glucose (a measure of glycolysis) on Day 7 only. The results show that 10 microM DNP stimulates oxidative and glycolytic metabolism in Day-5 to Day-7 cattle embryos, but this does not fully explain the observed increase in developmental competence. We propose that partial inhibition or uncoupling of oxidative phosphorylation may reduce the level of intracellular reactive oxygen species production, thereby facilitating development.|
|Keywords:||Blastocyst; Animals; Cattle; Tritium; Carbon Radioisotopes; Pyruvic Acid; 2,4-Dinitrophenol; Glucose; Adenosine Triphosphate; Uncoupling Agents; Fertilization in Vitro; Culture Techniques; Energy Metabolism; Glycolysis; Embryo, Mammalian|
|Appears in Collections:||Obstetrics and Gynaecology publications|
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