Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Maternal tract factors contribute to paternal seminal fluid impact on metabolic phenotype in offspring
Author: Bromfield, J.
Schjenken, J.
Chin, P.
Care, A.
Jasper, M.
Robertson, S.
Citation: PNAS, 2014; 111(6):2200-2205
Publisher: National Academy of Sciences
Issue Date: 2014
ISSN: 1091-6490
Statement of
John J. Bromfield, John E. Schjenken, Peck Y. Chin, Alison S. Care, Melinda J. Jasper and Sarah A. Robertson
Abstract: Paternal characteristics and exposures influence physiology and disease risks in progeny, but the mechanisms are mostly unknown. Seminal fluid, which affects female reproductive tract gene expression as well as sperm survival and integrity, provides one potential pathway. We evaluated in mice the consequences for offspring of ablating the plasma fraction of seminal fluid by surgical excision of the seminal vesicle gland. Conception was substantially impaired and, when pregnancy did occur, placental hypertrophy was evident in late gestation. After birth, the growth trajectory and metabolic parameters of progeny were altered, most profoundly in males, which exhibited obesity, distorted metabolic hormones, reduced glucose tolerance, and hypertension. Altered offspring phenotype was partly attributable to sperm damage and partly to an effect of seminal fluid deficiency on the female tract, because increased adiposity was also evident in adult male progeny when normal two-cell embryos were transferred to females mated with seminal vesicle-excised males. Moreover, embryos developed in female tracts not exposed to seminal plasma were abnormal from the early cleavage stages, but culture in vitro partly alleviated this. Absence of seminal plasma was accompanied by down-regulation of the embryotrophic factors Lif, Csf2, Il6, and Egf and up-regulation of the apoptosis-inducing factor Trail in the oviduct. These findings show that paternal seminal fluid composition affects the growth and health of male offspring, and reveal that its impact on the periconception environment involves not only sperm protection but also indirect effects on preimplantation embryos via oviduct expression of embryotrophic cytokines.
Keywords: Embryo development; programming; metabolic disorder; fertility; growth factors
Description: This article contains supporting information online at
Rights: © 2014 National Academy of Sciences. Freely available online through the PNAS open access option.
RMID: 0030007303
DOI: 10.1073/pnas.1305609111
Appears in Collections:Paediatrics publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.