Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/87057
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Type: Journal article
Title: Development of a robust protocol for gene expression analysis using formalin-fixed, paraffin-embedded liver transplant biopsy specimens
Author: Thompson, E.
Burt, A.
Barker, C.
Kirby, J.
Brain, J.
Citation: Journal of Clinical Pathology, 2013; 66(9):815-818
Publisher: BMJ Publishing Group
Issue Date: 2013
ISSN: 0021-9746
1366-8714
Statement of
Responsibility: 
Emily Thompson, Alastair D Burt, Catriona E Barker, John A Kirby, John G Brain
Abstract: Liver transplant biopsies are routinely archived following formalin fixation and paraffin embedding and may provide an additional source of diagnostic information following transcriptomic biomarker analysis. This study was designed to compare gene transcription between resting and stressed biliary cells in culture, these cells after fixation and embedding and archival liver transplant biopsy tissue. The transcription of p21/WAF1 and transforming growth factor (TGF)-β1 showed similar changes in the fresh and embedded liver cells. However, the expression of TGF-β2 was markedly different between the fresh and embedded samples, suggesting that fixation can produce sequence-specific artefacts. Sufficient quantities of pure RNA were recovered from all the liver transplant biopsies to allow complementary DNA production. Measurement of the transcription of all three genes showed variability between the cases. Although the results for individual transcripts should be interpreted with care, these data do suggest the feasibility of performing a larger biomarker discovery studies using archival tissue.
Keywords: Liver; Humans; Formaldehyde; Liver Transplantation; Paraffin Embedding; Tissue Fixation; Gene Expression Profiling; Gene Expression
Rights: This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 3.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/3.0/
RMID: 0030007699
DOI: 10.1136/jclinpath-2013-201548
Appears in Collections:Medicine publications

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