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Type: Book chapter
Title: Imaging blood vessels in the zebrafish
Author: Kamei, M.
Isogai, S.
Pan, W.
Weinstein, B.M.
Citation: The Zebrafish: Cellular and Developmental Biology, Part A, 2010 / Detrich, H.W., Westerfield, M., Zon, L.I. (ed./s), vol.100, Ch.2, pp.27-54
Publisher: Elsevier
Issue Date: 2010
Series/Report no.: Methods in Cell Biology; 100
ISBN: 9780123848925
Editor: Detrich, H.W.
Westerfield, M.
Zon, L.I.
Statement of
Makoto Kamei, Sumio Isogai, Weijun Pan, and Brant M. Weinstein
Abstract: Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.
Keywords: Blood Vessels
Embryo, Nonmammalian
Animals, Genetically Modified
In Situ Hybridization
Time-Lapse Imaging
Rights: Copyright © 2010 Elsevier Inc. All rights reserved.
DOI: 10.1016/B978-0-12-384892-5.00002-5
Appears in Collections:Aurora harvest 7
Paediatrics publications

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