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https://hdl.handle.net/2440/8759
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Type: | Journal article |
Title: | Evidence that nitric oxide stimulates feeding in the marsupial Sminthopsis crassicaudata |
Author: | Vozzo, R. Wittert, G. Chapman, I. Fraser, R. Hope, P. Horowitz, M. Alshaher, M. Kumar, V. Morley, J. |
Citation: | Comparative Biochemistry and Physiology C: Toxicology and Pharmacology, 1999; 123(2):145-151 |
Publisher: | PERGAMON-ELSEVIER SCIENCE LTD |
Issue Date: | 1999 |
ISSN: | 1532-0456 |
Statement of Responsibility: | Rosalie Vozzo; Gary A Wittert; Ian M Chapman; Robert Fraser; Perdita J Hope; Michael Horowitz; Motaz M Alshaher; Vijaya B Kumar; John E Morley |
Abstract: | Nitric oxide (NO) synthase inhibitors reduce food intake in rodents and chickens, suggesting that NO may stimulate feeding. We used two competitive, non-selective inhibitors of NO synthase (NOS), (NG-monomethyl-L-arginine ester [L-NMMA] and NG-nitro-L-arginine methyl ester [L-NAME]), to evaluate the role of NO mechanisms in the control of food intake in a marsupial model previously used in studies of appetite regulation. Adult male Sminthopsis crassicaudata (n = 11-16, 15 +/- 0.3 g, mean +/- S.E.M.) received L-NMMA (50, 100, 200 and 1000 mg/kg), L-NAME (50, 100 and 200 mg/kg), L-arginine (L-arg) the precursor of NO (1000 and 2000 mg/kg), L-NAME (200 mg/kg) in combination with L-arg (2000 mg/kg), or saline (0.9%). All drugs were administered intraperitoneally after 24 h of food deprivation, after which food was immediately made available ad libitum. Food intake was measured 0, 0.5, 1, 2, 4 and 24 h after treatments. In addition, we studied the effect of acute L-NAME administration on hypothalamic, cortical, hepatic and cardiac NOS activity by quantifying citrulline production. L-NMMA (1000 mg/kg) and L-NAME (100 and 200 mg/kg) suppressed food intake by 25%, 21%, and 30%, respectively, over 24 h after treatments (P < 0.05). L-arg (1000 and 2000 mg/kg) by itself had no significant effect on food intake when compared with saline (P > 0.05). When administered in combination with L-NAME (200 mg/kg), L-arg (2000 mg/kg) reversed L-NAME induced suppression of appetite (P> 0.05). Furthermore, L-NAME (200 mg/kg) significantly decreased hypothalamic (P < 0.01), cortical (P < 0.01) and hepatic (P < 0.03) NOS activity. L-NAME had no effect on cardiac NOS activity (P> 0.05). These data show that peripheral administration of L-NAME has a significant central effect, particularly in brain areas involved in appetite regulation, and suggest in marsupials, as in other mammals and birds, that NO plays a role in the regulation of food intake. |
Keywords: | Animals Marsupialia Nitric Oxide NG-Nitroarginine Methyl Ester omega-N-Methylarginine Feeding Behavior Male Nitric Oxide Synthase |
DOI: | 10.1016/S0742-8413(99)00022-5 |
Published version: | http://dx.doi.org/10.1016/s0742-8413(99)00022-5 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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