Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/87740
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Type: Journal article
Title: Inflammasome gene expression alterations in Staphylococcus aureus biofilm-associated chronic rhinosinusitis
Author: Jardeleza, C.
Miljkovic, D.
Baker, L.
Boase, S.
Tan, N.
Koblar, S.
Zalewski, P.
Rischmueller, M.
Lester, S.
Drilling, A.
Jones, D.
Tan, L.
Wormald, P.
Vreugde, S.
Citation: Rhinology, 2013; 51(4):315-322
Publisher: International Rhinologic Society
Issue Date: 2013
ISSN: 0300-0729
1996-8604
Statement of
Responsibility: 
C. Jardeleza, D. Miljkovic, L. Baker, S. Boase, N.C.W. Tan, S.A. Koblar, P. Zalewski, M. Rischmueller, S. Lester, A. Drilling, D. Jones, L.W. Tan, P.J. Wormald, S. Vreugde
Abstract: BACKGROUND: The role of inflammasomes in chronic inflammation has been the subject of intense research in recent years. Chronic rhinosinusitis (CRS), a persistent inflammatory disease, continues to be investigated hoping that a clearer pathophysiologic description will guide discovery of future treatment modalities. This study investigates the role of inflammasome complexes in CRS patients with Staphylococcus aureus biofilm infection, a key culprit associated with disease severity and recalcitrance. METHODOLOGY: Sinonasal tissue samples were collected from CRS patients with (P+) and without (P-) polyps and controls. S. aureus biofilm status was obtained using fluorescence in situ hybridization and classified as biofilm positive (B+) or negative (B-). RNA was analysed using a Human Inflammasome PCR array, profiling the expression of 84 genes involved in inflammasome function. RESULTS: Sixteen samples were obtained: 5 B+P+, 5 B-P- and 6 controls. Comparing B+P+ vs. controls showed the greatest number of differentially expressed genes. In particular, Absent in Melanoma 2 (AIM2) was consistently and significantly up-regulated in the B+P+ vs. B-P- and controls. In contrast, when comparing the B-P- vs. controls, no genes showed significant changes. CONCLUSION: Our results indicate the involvement of inflammasome complexes and their signalling pathways in CRS patients with polyps and S. aureus biofilms. In particular, AIM2, activated by intracellular double-stranded DNA, is up-regulated in this group, implying that S. aureus may play a role in intracellular triggering of the inflammasome response. Studies with further patient stratification and assessing corresponding protein expression are needed to further characterize the role of inflammasomes in CRS.
Keywords: Humans; Biofilms; Staphylococcus aureus; Staphylococcal Infections; Sinusitis; Rhinitis; Nasal Polyps; Chronic Disease; RNA, Messenger; Case-Control Studies; Adult; Aged; Middle Aged; Female; Male; Inflammasomes
Rights: Copyright status unknown
RMID: 0020134250
DOI: 10.4193/Rhino13.045
Published version: http://www.rhinologyjournal.com/abstract.php?id=1170
Appears in Collections:Medicine publications

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