Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Activating point mutations in the common beta subunit of the human GM-CSF, IL-3 and IL-5 receptors suggest the involvement of beta subunit dimerization and cell type-specific molecules in signalling
Author: Jenkins, B.
D'Andrea, R.
Gonda, T.
Citation: The EMBO Journal, 1995; 14(17):4276-4287
Publisher: IRL Press
Issue Date: 1995
ISSN: 0261-4189
Statement of
Jenkins, B J ; D'andrea, R ; Gonda, T J
Abstract: We have combined retroviral expression cloning with random mutagenesis to identify two activating point mutations in the common signal-transducing subunit (h beta c) of the receptors for human granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-3 and IL-5 by virtue of their ability to confer factor independence on the haemopoietic cell line, FDC-P1. One mutation (V449E) is located within the transmembrane domain and, by analogy with a similar mutation in the neu oncogene, may act by inducing dimerization of h beta c. The other mutation (I374N) lies in the extracellular, membrane-proximal portion of h beta c. Neither of these mutants, nor a previously described mutant of h beta c (FI delta, which has a small duplication in the extracellular region), was capable of inducing factor independence in CTLL-2 cells, while only V449E could induce factor independence in BAF-B03 cells. These results imply that the extracellular and transmembrane mutations act by different mechanisms. Furthermore, they imply that the mutants, and hence also wild-type h beta c, interact with cell type-specific signalling molecules. Models are presented which illustrate how these mutations may act and predict some of the characteristics of the putative receptor-associated signalling molecules.
Keywords: Hematopoietic Stem Cells
Cell Line
Cell Transformation, Neoplastic
Macromolecular Substances
Receptors, Granulocyte-Macrophage Colony-Stimulating Factor
Receptors, Interleukin-3
Receptors, Interleukin
Recombinant Proteins
DNA Primers
Mutagenesis, Site-Directed
Polymerase Chain Reaction
Signal Transduction
Cell Division
Gene Expression Regulation
Amino Acid Sequence
Base Sequence
Point Mutation
Molecular Sequence Data
Receptors, Interleukin-5
DOI: 10.1002/j.1460-2075.1995.tb00102.x
Published version:
Appears in Collections:Aurora harvest 4
Medicine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.