Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/88257
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Abdelrahman, A. | - |
dc.contributor.author | Ornatsky, O. | - |
dc.contributor.author | Bandura, D. | - |
dc.contributor.author | Baranov, V. | - |
dc.contributor.author | Kinach, R. | - |
dc.contributor.author | Dai, S. | - |
dc.contributor.author | Thickett, S. | - |
dc.contributor.author | Tanner, S. | - |
dc.contributor.author | Winnik, M. | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Journal of Analytical Atomic Spectrometry, 2010; 25(3):260-268 | - |
dc.identifier.issn | 0267-9477 | - |
dc.identifier.issn | 1364-5544 | - |
dc.identifier.uri | http://hdl.handle.net/2440/88257 | - |
dc.description.abstract | We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells. | - |
dc.description.statementofresponsibility | Ahmed I. Abdelrahman, Olga Ornatsky, Dmitry Bandura, Vladimir Baranov, Robert Kinach, Sheng Dai, Stuart C. Thickett, Scott Tanner and Mitchell A. Winnik | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.rights | ©The Royal Society of Chemistry 2010 | - |
dc.source.uri | http://dx.doi.org/10.1039/b921770c | - |
dc.title | Metal-containing polystyrene beads as standards for mass cytometry | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1039/b921770c | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 2 Chemistry publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.