Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/8827
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Type: Journal article
Title: The vascular endothelial growth factor mRNA contains an internal ribosome entry site
Author: Miller, D.
Dibbens, J.
Damert, A.
Risau, W.
Vadas, M.
Goodall, G.
Citation: FEBS Letters, 1998; 434(3):417-420
Publisher: ELSEVIER SCIENCE BV
Issue Date: 1998
ISSN: 0014-5793
1873-3468
Abstract: Vascular endothelial growth factor (VEGF), an essential regulator of angiogenesis during early development as well as during the growth of solid tumours, bears an unusually large 5' untranslated region (5'-UTR) in the mRNA of over 1000 nucleotides. We found that the VEGF 5'-UTR, despite being GC-rich and containing an upstream short open reading frame, promotes efficient translation of a luciferase reporter. The VEGF 5'-UTR also allowed translation of luciferase from a dicistronic mRNA when placed between the two cistrons, demonstrating that it contains an internal ribosome entry site. Deletion analysis indicated that the IRES resides towards the 3' end of the 5'-UTR.
Keywords: 3T3 Cells; Ribosomes; Animals; Mice, Inbred BALB C; Mice; Vascular Endothelial Growth Factors; Vascular Endothelial Growth Factor A; Endothelial Growth Factors; RNA, Messenger; DNA Primers; Lymphokines; Protein Biosynthesis; Base Sequence
RMID: 0030004960
DOI: 10.1016/S0014-5793(98)01025-4
Appears in Collections:Medicine publications

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