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https://hdl.handle.net/2440/8926
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Type: | Journal article |
Title: | A novel activating anti-β1 integrin monoclonal antibody binds to the cysteine-rich repeats in the β1 chain |
Other Titles: | A novel activating anti-beta1 integrin monoclonal antibody binds to the cysteine-rich repeats in the beta1 chain |
Author: | Faull, R. Wang, J. Leavesley, D. Puzon, W. Russ, G. Vestweber, D. Takada, Y. |
Citation: | Journal of Biological Chemistry, 1996; 271(41):25099-25106 |
Publisher: | American Society for Biochemistry and Molecular Biology |
Issue Date: | 1996 |
ISSN: | 1083-351X 1083-351X |
Statement of Responsibility: | Randall J. Faull, Jian Wang, David I. Leavesley, Wilma Puzon, Graeme R. Russ, Dietmar Vestweber and Yoshikazu Takada |
Abstract: | The functional status of an integrin depends on the conformation of its extracellular domain, which is controlled by the cell expressing that receptor. The transmission of regulatory signals from within the cell is considered to be via propagated conformational changes from the receptor's cytoplasmic tails to the extracellular ligand binding “pocket.” The end result is increased accessibility of the ligand binding pocket in the high affinity (“active”) form of integrins. We report a novel monoclonal antibody (QE.2E5) that binds within the cysteine-rich repeats in the integrin β1 chain and induces high affinity binding of fibronectin to the integrin α5β1. The QE.2E5 epitope is located approximately 200 residues both from the predicted binding site for fibronectin and from the epitopes recognized by other activating anti-β1 monoclonal antibodies. It is also expressed on β1 integrins from a number of nonhuman species. Although they have the same functional effects, the binding of QE.2E5 and another activating antibody (8A2) to the receptor have contrasting effects on the expression of an activation-dependent epitope in the β1 chain. We propose that the cysteine-rich repeats contain a regulatory region that is distinct from those previously described in the integrin β1 chain. |
Keywords: | Endothelium, Vascular Umbilical Veins Cell Line Tumor Cells, Cultured Animals Mice, Inbred BALB C Humans Mice Cysteine Fibronectins Recombinant Fusion Proteins Antibodies, Monoclonal Binding Sites, Antibody Epitopes Ligands Cell Adhesion Signal Transduction Binding Sites Amino Acid Sequence Integrin beta1 |
Rights: | © 1996 by The American Society for Biochemistry and Molecular Biology, Inc. |
DOI: | 10.1074/jbc.271.41.25099 |
Published version: | http://dx.doi.org/10.1074/jbc.271.41.25099 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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