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Type: Journal article
Title: Dysregulated hematopoiesis and a progressive neurological disorder induced by expression of an activated form of the human common β chain in transgenic mice
Other Titles: Dysregulated hematopoiesis and a progressive neurological disorder induced by expression of an activated form of the human common beta chain in transgenic mice
Author: D'Andrea, R.
Harrison-Findik, D.
Butcher, C.
Finnie, J.
Blumbergs, P.
Bartley, P.
McCormack, M.
Jones, K.
Rowland, R.
Gonda, T.
Vadas, M.
Citation: Journal of Clinical Investigation, 1998; 102(11):1951-1960
Issue Date: 1998
ISSN: 0021-9738
Statement of
Richard J. D'Andrea, Duygu Harrison-Findik, Carolyn M. Butcher, John Finnie, Peter Blumbergs, Paul Bartley, Matthew McCormack, Karen Jones, Robert Rowland, Thomas J. Gonda and Mathew A. Vadas
Abstract: Previously we described activating mutations of hbetac, the common signaling subunit of the receptors for the hematopoietic and inflammatory cytokines, GM-CSF, IL-3, and IL-5. The activated mutant, hbetacFIDelta, is able to confer growth factor-independent proliferation on the murine myeloid cell line FDC-P1, and on primary committed myeloid progenitors. We have used this activating mutation to study the effects of chronic cytokine receptor stimulation. Transgenic mice were produced carrying the hbetacFIDelta cDNA linked to the constitutive promoter derived from the phosphoglycerate kinase gene, PGK-1. Transgene expression was demonstrated in several tissues and functional activity of the mutant receptor was confirmed in hematopoietic tissues by the presence of granulocyte macrophage and macrophage colony-forming cells (CFU-GM and CFU-M) in the absence of added cytokines. All transgenic mice display a myeloproliferative disorder characterized by splenomegaly, erythrocytosis, and granulocytic and megakaryocytic hyperplasia. This disorder resembles the human disease polycythemia vera, suggesting that activating mutations in hbetac may play a role in the pathogenesis of this myeloproliferative disorder. In addition, these transgenic mice develop a sporadic, progressive neurological disease and display bilateral, symmetrical foci of necrosis in the white matter of brain stem associated with an accumulation of macrophages. Thus, chronic hbetac activation has the potential to contribute to pathological events in the central nervous system.
Keywords: Brain Stem
Mice, Inbred BALB C
Mice, Inbred CBA
Mice, Transgenic
Neurodegenerative Diseases
Myeloproliferative Disorders
Polycythemia Vera
Disease Models, Animal
Disease Progression
Receptors, Cell Surface
Recombinant Fusion Proteins
Gene Expression Regulation
Cytokine Receptor Common beta Subunit
Rights: Copyright © 1998, The American Society for Clinical Investigation.
DOI: 10.1172/JCI3729
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