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Type: Journal article
Title: Impaired glucose metabolism in response to high fat diet in female mice conceived by In Vitro Fertilization (IVF) or ovarian stimulation alone
Author: Chen, M.
Wu, L.
Wu, F.
Wittert, G.
Norman, R.
Robker, R.
Heilbronn, L.
Citation: PLoS One, 2014; 9(11):e113155-1-e113155-9
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
Statement of
Miaoxin Chen, Linda Wu, Fang Wu, Gary A. Wittert, Robert J. Norman, Rebecca L. Robker, Leonie K. Heilbronn
Abstract: Individuals conceived by in vitro fertilization (IVF) may be at increased risk of cardio-metabolic disorders. We recently reported that IVF conceived male mice displayed impaired glucose metabolism at normal and high body weights. In this study, we examined glucose metabolism in mature female C57BL/6J mice that were conceived by natural conception (NC), by ovarian stimulation (OS) or by IVF following chow or high-fat diet (HFD) for 8 weeks. By design, litter size was comparable between groups, but interestingly the birth weight of IVF and OS females was lower than NC females (p≤0.001). Mature IVF female mice displayed increased fasting glucose as compared to NC and OS mice, irrespective of diet. Mature IVF and OS mice were also more susceptible to the metabolic consequences of high fat diet as compared with NC females, with impaired glucose tolerance (p≤0.01), whereas peripheral insulin resistance and increased hepatic expression of gluconeogenic genes Ppargc1α, Pck1 and G6pc was observed in IVF mice only (p<0.05). This study suggests that ovarian stimulation alone and IVF program distinct metabolic effects in females, but that high fat diet may be required to unmask these effects. This study adds to the growing body of literature that assisted reproduction procedures may increase the risk of developing type 2 diabetes in an obesity prone environment.
Keywords: Animals
Mice, Inbred C57BL
Glucose Metabolism Disorders
Insulin Resistance
Birth Weight
DNA, Mitochondrial
Blotting, Western
Fertilization in Vitro
Ovulation Induction
Energy Metabolism
Real-Time Polymerase Chain Reaction
Diet, High-Fat
Rights: © 2014 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0113155
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