Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/90508
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Type: Journal article
Title: Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
Author: Christo, S.
Diener, K.
Nordon, R.
Brown, M.
Griesser, H.
Vasilev, K.
Christo, F.
Hayball, J.
Citation: Scientific Reports, 2015; 5(1):7760-1-7760-8
Publisher: Nature Publishing Group
Issue Date: 2015
ISSN: 2045-2322
2045-2322
Statement of
Responsibility: 
Susan N. Christo, Kerrilyn R. Diener, Robert E. Nordon, Michael P. Brown, Hans J. Griesser, Krasimir Vasilev, Farid C. Christo, John D. Hayball
Abstract: The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.
Keywords: T-Lymphocytes; Animals; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; Ligands; Lymphocyte Activation; Calcium Signaling; Fluorescence; CD3 Complex; CD28 Antigens
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
RMID: 0030020684
DOI: 10.1038/srep07760
Appears in Collections:Paediatrics publications

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