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https://hdl.handle.net/2440/91795
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Type: | Journal article |
Title: | Applicability of plasmid calibrant pTC1507 in quantification of TC1507 maize: an interlaboratory study |
Author: | Meng, Y. Liu, X. Wang, S. Zhang, D. Yang, L. |
Citation: | Journal of Agricultural and Food Chemistry, 2012; 60(1):23-28 |
Publisher: | AMER CHEMICAL SOC |
Issue Date: | 2012 |
ISSN: | 0021-8561 1520-5118 |
Statement of Responsibility: | Yanan Meng, Xin Liu, Shu Wang, Dabing Zhang, and Litao Yang |
Abstract: | To enforce the labeling regulations of genetically modified organisms (GMOs), the application of DNA plasmids as calibrants is becoming essential for the practical quantification of GMOs. This study reports the construction of plasmid pTC1507 for a quantification assay of genetically modified (GM) maize TC1507 and the collaborative ring trial in international validation of its applicability as a plasmid calibrant. pTC1507 includes one event-specific sequence of TC1507 maize and one unique sequence of maize endogenous gene zSSIIb. A total of eight GMO detection laboratories worldwide were invited to join the validation process, and test results were returned from all eight participants. Statistical analysis of the returned results showed that real-time PCR assays using pTC1507 as calibrant in both GM event-specific and endogenous gene quantifications had high PCR efficiency (ranging from 0.80 to 1.15) and good linearity (ranging from 0.9921 to 0.9998). In a quantification assay of five blind samples, the bias between the test values and true values ranged from 2.6 to 24.9%. All results indicated that the developed pTC1507 plasmid is applicable for the quantitative analysis of TC1507 maize and can be used as a suitable substitute for dried powder certified reference materials (CRMs). |
Keywords: | genetically modified maize collaborative ring trial plasmid calibrant pTC1507 |
Rights: | © 2011 American Chemical Society |
DOI: | 10.1021/jf2034972 |
Published version: | http://dx.doi.org/10.1021/jf2034972 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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