Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/92449
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Type: Journal article
Title: GMO detection in food and feed through screening by visual loop-mediated isothermal amplification assays
Author: Wang, C.
Li, R.
Quan, S.
Shen, P.
Zhang, D.
Shi, J.
Yang, L.
Citation: Analytical and Bioanalytical Chemistry, 2015; 407(16):4829-4834
Publisher: Springer Verlag
Issue Date: 2015
ISSN: 1618-2650
1618-2650
Statement of
Responsibility: 
Cong Wang, Rong Li, Sheng Quan, Ping Shen, Dabing Zhang, Jianxin Shi, Litao Yang
Abstract: Isothermal DNA/RNA amplification techniques are the primary methodology for developing on-spot rapid nucleic acid amplification assays, and the loop-mediated isothermal amplification (LAMP) technique has been developed and applied in the detection of foodborne pathogens, plant/animal viruses, and genetically modified (GM) food/feed contents. In this study, one set of LAMP assays targeting on eight frequently used universal elements, marker genes, and exogenous target genes, such as CaMV35S promoter, FMV35S promoter, NOS, bar, cry1Ac, CP4 epsps, pat, and NptII, were developed for visual screening of GM contents in plant-derived food samples with high efficiency and accuracy. For these eight LAMP assays, their specificity was evaluated by testing commercial GM plant events and their limits of detection were also determined, which are 10 haploid genome equivalents (HGE) for FMV35S promoter, cry1Ac, and pat assays, as well as five HGE for CaMV35S promoter, bar, NOS terminator, CP4 epsps, and NptII assays. The screening applicability of these LAMP assays was further validated successfully using practical canola, soybean, and maize samples. The results suggested that the established visual LAMP assays are applicable and cost-effective for GM screening in plant-derived food samples.
Keywords: Genetically modifiedorganism; Loop-mediated isothermalamplification; Screening test
Rights: © Springer-Verlag Berlin Heidelberg 2015
RMID: 0030026695
DOI: 10.1007/s00216-015-8652-z
Appears in Collections:Agriculture, Food and Wine publications

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