Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/93018
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Immunization of fucose-containing polysaccharides from Reishi mushroom induces antibodies to tumor-associated Globo H-series epitopes
Author: Liao, S.
Liang, C.
Ho, M.
Hsu, T.
Tsai, T.
Hsieh, Y.
Tsai, C.
Li, S.
Cheng, Y.
Tsao, S.
Lin, T.
Lin, Z.
Yang, W.
Ren, C.
Lin, K.
Khoo, K.
Lin, C.
Hsu, H.
Wu, C.
Wong, C.
Citation: Proceedings of the National Academy of Sciences of USA, 2013; 110(34):13809-13814
Publisher: National Academy of Sciences
Issue Date: 2013
ISSN: 0027-8424
1091-6490
Statement of
Responsibility: 
Shih-Fen Liao, Chi-Hui Liang, Ming-Yi Ho, Tsui-Ling Hsu, Tsung-I Tsai, Yves S.-Y. Hsieh, Chih-Ming Tsai, Shiou-Ting Li, Yang-Yu Cheng, Shu-Ming Tsao, Tung-Yi Lin, Zong-Yan Lin, Wen-Bin Yang, Chien-Tai Ren, Kuo-I Lin, Kay-Hooi Khoo, Chun-Hung Lin, Hsien-Yeh Hsu, Chung-Yi Wu, and Chi-Huey Wong
Abstract: Carbohydrate-based vaccines have shown therapeutic efficacy for infectious disease and cancer. The mushroom Ganoderma lucidum (Reishi) containing complex polysaccharides has been used as antitumor supplement, but the mechanism of immune response has rarely been studied. Here, we show that the mice immunized with a l-fucose (Fuc)-enriched Reishi polysaccharide fraction (designated as FMS) induce antibodies against murine Lewis lung carcinoma cells, with increased antibody-mediated cytotoxicity and reduced production of tumor-associated inflammatory mediators (in particular, monocyte chemoattractant protein-1). The mice showed a significant increase in the peritoneal B1 B-cell population, suggesting FMS-mediated anti-glycan IgM production. Furthermore, the glycan microarray analysis of FMS-induced antisera displayed a high specificity toward tumor-associated glycans, with the antigenic structure located in the nonreducing termini (i.e., Fucα1-2Galβ1-3GalNAc-R, where Gal, GalNAc, and R represent, respectively, D-galactose, D-N-acetyl galactosamine, and reducing end), typically found in Globo H and related tumor antigens. The composition of FMS contains mainly the backbone of 1,4-mannan and 1,6-α-galactan and through the Fucα1-2Gal, Fucα1-3/4Man, Fucα1-4Xyl, and Fucα1-2Fuc linkages (where Man and Xyl represent d-mannose and d-xylose, respectively), underlying the molecular basis of the FMS-induced IgM antibodies against tumor-specific glycans.
Keywords: mushroom polysaccharide
antitumor activity
anti-Globo H antibody
Rights: © National Academy of Sciences
DOI: 10.1073/pnas.1312457110
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 2

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.