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Type: Journal article
Title: Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9
Author: O'Reilly, L.
Ekert, P.
Harvey, N.
Marsden, V.
Cullen, L.
Vaux, D.
Hacker, G.
Magnusson, C.
Pakusch, M.
Cecconi, F.
Kuida, K.
Strasser, A.
Huang, D.
Kumar, S.
Citation: Cell Death and Differentiation, 2002; 9(8):832-841
Publisher: Nature Publishing Group
Issue Date: 2002
ISSN: 1350-9047
Abstract: We have generated rat monoclonal antibodies that specifically recognise caspase-2 from many species, including mouse, rat and humans. Using these antibodies, we have investigated caspase-2 expression, subcellular localisation and processing. We demonstrate that caspase-2 is expressed in most tissues and cell types. Cell fractionation and immunohistochemistry experiments show that caspase-2 is found in the nuclear and cytosolic fractions, including a significant portion present in the Golgi complex. We found that caspase-2 is processed in response to many apoptotic stimuli but experiments with caspase-2 deficient mice demonstrated that it is not required for apoptosis of thymocytes or dorsal root ganglia (DRG) neurons in response to a variety of cytotoxic stimuli. Caspase-2 processing does not occur in thymocytes lacking Apaf-1 or caspase-9, suggesting that in this cell type, activation of caspase-2 occurs downstream of apoptosome formation.
Keywords: Ganglia, Spinal
Hela Cells
Cell Nucleus
Golgi Apparatus
Mice, Inbred C57BL
Mice, Knockout
Rats, Wistar
Antibodies, Monoclonal
Signal Transduction
Antibody Specificity
Gene Expression Regulation, Enzymologic
Caspase 9
Apoptotic Protease-Activating Factor 1
Caspase 2
DOI: 10.1038/sj.cdd.4401033
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