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Type: Journal article
Title: Cloning of leptin cDNA and assignment to the long arm of chromosome 5 in the marsupial Sminthopsis crassicaudata
Author: Hope, P.
Webb, G.
Lok, S.
Hope, R.
Turnbull, H.
Jelmberg, A.
Wittert, G.
Citation: Cytogenetic and Genome Research, 2000; 90(1-2):22-29
Publisher: Karger
Issue Date: 2000
ISSN: 1424-8581
Abstract: We have isolated and sequenced full-length cDNA clones for leptin in the dasyurid marsupial Sminthopsis crassicaudata (fat-tailed dunnart). Southern and in situ hybridisation data indicated a single leptin gene in the S. crassicauda- ta genome, localised to arbitrary chromosome bands 5q24--> q31 on the long arm of chromosome 5, the short-arm terminus of which bears the only nucleolar organising region. The nucleotide sequence of the cDNAs revealed that the primary translation product of S. crassicaudata leptin is composed of 167 amino acid residues, with a potential signal peptide of 21 residues. The mature protein of 146 amino acids is 82% similar to both the mouse and human proteins and is predicted to have a molecular weight of 16.26 kDa. Northern blot analysis revealed that the corresponding mRNA is approximately 3.9 kb in size and is expressed only in white adipose tissue of this marsupial species. Evolutionary analyses indicate that S. crassicaudata leptin cDNA has evolved at a significantly faster rate than cDNAs from eutherian mammals.
Keywords: Animals
RNA, Messenger
Blotting, Southern
In Situ Hybridization, Fluorescence
Physical Chromosome Mapping
Cloning, Molecular
Sequence Alignment
Amino Acid Sequence
Base Sequence
Molecular Sequence Data
DOI: 10.1159/000015655
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Appears in Collections:Aurora harvest
Medicine publications

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