Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/94010
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Laspiur, J. | - |
dc.contributor.author | Burton, J. | - |
dc.contributor.author | Weber, P. | - |
dc.contributor.author | Moore, J. | - |
dc.contributor.author | Kirkwood, R. | - |
dc.contributor.author | Trottier, N. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | The Journal of Nutrition, 2009; 139(9):1677-1684 | - |
dc.identifier.issn | 0022-3166 | - |
dc.identifier.issn | 1541-6100 | - |
dc.identifier.uri | http://hdl.handle.net/2440/94010 | - |
dc.description.abstract | To test the hypothesis that under restricted and surfeit protein intake the mammary gland undergoes adaptive regulation, changes in mammary tissue mRNA abundance of cationic amino acid (AA) transporter (CAT)-1, CAT-2B, alanine/serine/cysteine/threonine transporter 1 (ASCT1), and broad specificity transporter for neutral and cationic AA (ATB⁰,⁺), and CAT-1 protein abundance were investigated at 2 stages of lactation. Eighteen sows were allocated to a 2 × 3 randomized incomplete block design with 2 stages of lactation (early and peak) and 3 protein levels: deficient (D), adequate (A), or in excess (E) of lactation requirement. In early lactation, compared with A, sows fed E had lower (P = 0.05) piglet growth rate and sows fed D or E had lower (P ≤ 0.05) casein yield. In early lactation, piglet growth rate and milk protein and casein yield increased from D to A and decreased from A to E (quadratic, P = 0.095, P < 0.05, and P < 0.01, respectively). Protein intake did not affect CAT-1, ASCT1, ATB⁰,⁺ mRNA abundance, or CAT-1 protein level. Overall, CAT-2B mRNA abundance decreased linearly with increasing protein intake (P < 0.05). Compared with A, E decreased CAT-2B mRNA abundance (P < 0.05). Compared with early lactation, peak lactation did not increase CAT-1 mRNA abundance or relative CAT-1 protein content, but increased abundance of ASCT1 and ATB⁰,⁺ mRNA (P < 0.01). Mammary CAT-2B appears to be adaptively regulated in vivo at the transcription level, whereas ASCT1 and ATB⁰,⁺ mRNA abundances are associated only with stage of lactation. Neither protein intake nor stage of lactation affects porcine mammary CAT-1 gene expression in vivo. | - |
dc.description.statementofresponsibility | Juliana Pérez Laspiur, Jeanne L. Burton, Patty S. D. Weber, Julie Moore, Roy N. Kirkwood, and Nathalie L. Trottier | - |
dc.language.iso | en | - |
dc.publisher | American Society for Nutrition | - |
dc.rights | © 2009 American Society for Nutrition. | - |
dc.source.uri | http://dx.doi.org/10.3945/jn.108.103549 | - |
dc.subject | Mammary Glands, Animal | - |
dc.subject | Milk | - |
dc.subject | Animals | - |
dc.subject | Animals, Newborn | - |
dc.subject | Swine | - |
dc.subject | Protein Deficiency | - |
dc.subject | Weight Gain | - |
dc.subject | Amino Acids | - |
dc.subject | Amino Acid Transport Systems | - |
dc.subject | Dietary Proteins | - |
dc.subject | Caseins | - |
dc.subject | RNA, Messenger | - |
dc.subject | Diet | - |
dc.subject | Random Allocation | - |
dc.subject | Lactation | - |
dc.subject | Female | - |
dc.title | Dietary protein intake and stage of lactation differentially modulate amino acid transporter mRNA abundance in porcine mammary tissue | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.3945/jn.108.103549 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kirkwood, R. [0000-0002-3294-8301] | - |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest 2 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.