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Type: Journal article
Title: Diversity of aquatic pseudomonas species and their activity against the fish pathogenic oomycete saprolegnia
Author: Liu, Y.
Rzeszutek, E.
van der Voort, M.
Wu, C.
Thoen, E.
Skaar, I.
Bulone, V.
Dorrestein, P.
Raaijmakers, J.
de Bruijn, I.
Citation: PLoS One, 2015; 10(8):e0136241-1-e0136241-17
Publisher: Public Library of Science
Issue Date: 2015
ISSN: 1932-6203
Editor: Almeida, A.
Statement of
Yiying Liu, Elzbieta Rzeszutek, Menno van der Voort, Cheng-Hsuan Wu, Even Thoen, Ida Skaar, Vincent Bulone, Pieter C. Dorrestein, Jos M. Raaijmakers, Irene de Bruijn
Abstract: Emerging fungal and oomycete pathogens are increasingly threatening animals and plants globally. Amongst oomycetes, Saprolegnia species adversely affect wild and cultivated populations of amphibians and fish, leading to substantial reductions in biodiversity and food productivity. With the ban of several chemical control measures, new sustainable methods are needed to mitigate Saprolegnia infections in aquaculture. Here, PhyloChip-based community analyses showed that the Pseudomonadales, particularly Pseudomonas species, represent one of the largest bacterial orders associated with salmon eggs from a commercial hatchery. Among the Pseudomonas species isolated from salmon eggs, significantly more biosurfactant producers were retrieved from healthy salmon eggs than from Saprolegnia-infected eggs. Subsequent in vivo activity bioassays showed that Pseudomonas isolate H6 significantly reduced salmon egg mortality caused by Saprolegnia diclina. Live colony mass spectrometry showed that strain H6 produces a viscosin-like lipopeptide surfactant. This biosurfactant inhibited growth of Saprolegnia in vitro, but no significant protection of salmon eggs against Saprolegniosis was observed. These results indicate that live inocula of aquatic Pseudomonas strains, instead of their bioactive compound, can provide new (micro)biological and sustainable means to mitigate oomycete diseases in aquaculture.
Keywords: Animals
Fish Diseases
Water Microbiology
Base Sequence
Molecular Sequence Data
Rights: © 2015 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0136241
Appears in Collections:Agriculture, Food and Wine publications
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