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Type: Journal article
Title: The effect of chemically induced colitis, psychological stress and their combination on visceral pain in female Wistar rats
Author: Deiteren, A.
Vermeulen, W.
Moreels, T.
Pelckmans, P.
De Man, J.
De Winter, B.
Citation: Stress: the International Journal on the Biology of Stress, 2014; 17(5):431-444
Publisher: Informa Healthcare
Issue Date: 2014
ISSN: 1025-3890
Statement of
Annemie Deiteren, Wim Vermeulen, Tom G. Moreels, Paul A. Pelckmans, Joris G. De Man, and Benedicte Y. De Winter
Abstract: Visceral sensitivity is of pathophysiological importance in abdominal pain disorders and can be modulated by inflammation and stress. However, it is unclear whether inflammation and stress alter visceral perception independently of each other or in conjunction through neuroendocrine interactions. Therefore, we compared the short- and long-term effects of experimental colitis and water avoidance stress (WAS), alone or in combination, on visceral sensitivity in female Wistar rats. Colitis was induced by trinitrobenzene sulfonic acid (TNBS) and colonoscopically confirmed. During WAS, rats were placed on a platform surrounded by water for 1 h. Visceral sensitivity was assessed by quantifying the visceromotor responses (VMRs) to colorectal distension. Activation of the hypothalamic-pituitary-adrenal axis was determined by measuring serum corticosterone in a separate protocol. TNBS instillation resulted in overt colitis, associated with significant visceral hypersensitivity during the acute inflammatory phase (3 days post-TNBS; n = 8/group); after colitis had subsided (28 days post-TNBS), hypersensitivity was resolved (n = 4-8/group). Single WAS was associated with increased VMRs of a magnitude comparable to acute TNBS-induced hypersensitivity (n = 8/group). However, after repetitive WAS no significant hypersensitivity was present (n = 8/group). No additive effect of colitis and stress was seen on visceral pain perception (n = 6-8/group). Corticosterone levels were only increased in acute TNBS-colitis, acute WAS and their combination. To conclude, both colitis and stress successfully induced short-term visceral hypersensitivity and activated the hypothalamic-pituitary-adrenal axis, but long-term effects were absent. In addition, our current findings do not support an additive effect of colitis and stress on visceral sensitivity in female Wistar rats.
Keywords: Colorectal distension; inflammation; trinitrobenzene sulfonic acid; visceral hypersensitivity; visceromotor response; water avoidance stress
Rights: © 2014 Informa UK Ltd
RMID: 0030036978
DOI: 10.3109/10253890.2014.951034
Appears in Collections:Medicine publications

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