Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/95890
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Type: Journal article
Title: Performance characteristics of ELISA to detect bovine viral diarrhea virus (BVDV) antibodies using colostrum
Author: Jenvey, C.
Weir, A.
Reichel, M.
Cockcroft, P.
Citation: Open Journal of Veterinary Medicine, 2015; 5(02):35-41
Publisher: Scientific Research Publishing
Issue Date: 2015
ISSN: 2165-3356
2165-3364
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Responsibility: 
Caitlin J. Jenvey, Andrew M. Weir, Michael P. Reichel, Peter D. Cockcroft
Abstract: Colostrum contains substantially higher concentrations of immunoglobulins (Igs) when compared with serum or milk, which may improve the diagnostic sensitivity of an antibody ELISA when using colostrum. In this study, BVD was used as a model to identify the performance characteristics of colostrum and to assess the potential for increased ELISA sensitivity when compared with serum. Blood and colostrum samples were collected from cows within two dairy cattle herds: a previously infected and BVD-vaccinated Holstein-Friesian (positive herd) herd, and a bulk-tank milk antibody negative (negative herd) Jersey herd. All samples were tested using a commercial BVDV antibody ELISA. Median sample-to-positive (S/P) colostrum ratios were significantly higher than their respective serum counterparts, and positive herd S/P ratios were significantly higher than the respective negative herd values (P < 0.001). Using the manufacturer’s recommended serum dilution (1:5) and colostrum dilution (undiluted), and a cut-off threshold S/P ratio of 0.2, diagnostic sensitivity (DSe) and diagnostic specificity (DSp) for colostrum were 100% and 70%, respectively. These values increased to 100% DSe and 100% DSp with an increase in cut-off threshold S/P to 0.5. At a sample dilution of 1:100, the DSe of colostrum was 90% and significantly higher compared with serum (DSe 17%). Colostrum has the potential to improve identification of previously infected animals, either individually, or when using pooled samples.
Keywords: BVDV; Colostrum; ELISA; Sensitivity; Specificity
Rights: © 2015 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/
RMID: 0030029246
DOI: 10.4236/ojvm.2015.52006
Appears in Collections:Animal and Veterinary Sciences publications

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