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Type: Journal article
Title: Ion transport in broad bean leaf mesophyll under saline conditions
Author: Percey, W.
Shabala, L.
Breadmore, M.
Guijt, R.
Bose, J.
Shabala, S.
Citation: Planta, 2014; 240(4):729-743
Publisher: Springer
Issue Date: 2014
ISSN: 0032-0935
Statement of
William J. Percey, Lana Shabala, Michael C. Breadmore, Rosanne M. Guijt, Jayakumar Bose, Sergey Shabala
Abstract: Main conclusion Salt stress reduces the ability of mesophyll tissue to respond to light. Potassium outward rectifying channels are responsible for 84 % of Na⁺ induced potassium efflux from mesophyll cells. Modulation in ion transport of broad bean (Vicia faba L.) mesophyll to light under increased apoplastic salinity stress was investigated using vibrating ion-selective microelectrodes (the MIFE technique). Increased apoplastic Na⁺ significantly affected mesophyll cells ability to respond to light by modulating ion transport across their membranes. Elevated apoplastic Na⁺ also induced a significant K⁺ efflux from mesophyll tissue. This efflux was mediated predominately by potassium outward rectifying channels (84 %) and the remainder of the efflux was through nonselective cation channels. NaCl treatment resulted in a reduction in photosystem II efficiency in a dose- and time-dependent manner. In particular, reductions in Fv‘/Fm‘ were linked to K⁺ homeostasis in the mesophyll tissue. Increased apoplastic Na⁺ concentrations induced vanadate-sensitive net H⁺ efflux, presumably mediated by the plasma membrane H⁺-ATPase. It is concluded that the observed pump’s activation is essential for the maintenance of membrane potential and ion homeostasis in the cytoplasm of mesophyll under salt stress.
Keywords: Flux; H⁺-ATPase; membrane potential; potassium; photosynthesis
Rights: © Springer-Verlag Berlin Heidelberg 2014
RMID: 0030031561
DOI: 10.1007/s00425-014-2117-z
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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