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https://hdl.handle.net/2440/96515
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dc.contributor.author | Wang, K. | - |
dc.contributor.author | Brooks, D. | - |
dc.contributor.author | Thornburg, K. | - |
dc.contributor.author | Morrison, J. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | The Journal of Physiology, 2012; 590(21):5425-5437 | - |
dc.identifier.issn | 0022-3751 | - |
dc.identifier.issn | 1469-7793 | - |
dc.identifier.uri | http://hdl.handle.net/2440/96515 | - |
dc.description.abstract | In vitro studies using rat and fetal sheep cardiomyocytes indicate that, in addition to its role as a clearance receptor, the insulin-like growth factor 2 receptor (IGF-2R) can induce cardiomyocyte hypertrophy. In the present study, we have determined the effect of specific activation of the IGF-2R in the heart of the late gestation fetus on cardiomyocyte development. Leu(27)IGF-2, an IGF-2R agonist, was infused into the fetal left circumflex coronary artery for 4 days beginning at 128.1 ± 0.4 days gestation. Ewes were humanely killed at 132.2 ± 1.2 days gestation (term, 150 days). Fetuses were delivered and hearts dissected to isolate the cardiomyocytes and to collect and snap-freeze tissue. Leu(27)IGF-2 infusion into the left circumflex coronary artery of fetal sheep increased the area of binucleated cardiomyocytes in the left, but not the right, ventricle. However, this infusion of Leu(27)IGF-2 did not change fetal weight, heart weight, blood pressure, blood gases or cardiomyocyte proliferation/binucleation. The increase in cardiomyocyte size in the Leu(27)IGF-2-infused group was associated with increased expression of proteins in the Gαs, but not the Gαq, signalling pathway. We concluded that infusion of Leu(27)IGF-2 into the left circumflex coronary artery causes cardiac IGF-2R activation in the left ventricle of the heart, and this stimulates cardiomyocyte hypertrophy in a Gαs-dependent manner. | - |
dc.description.statementofresponsibility | Kimberley C.W.Wang, Doug A. Brooks, Kent L. Thornburg, and Janna L. Morrison | - |
dc.language.iso | en | - |
dc.publisher | Wiley | - |
dc.rights | © 2012 The Authors | - |
dc.source.uri | http://dx.doi.org/10.1113/jphysiol.2012.238410 | - |
dc.subject | Coronary Vessels | - |
dc.title | Activation of IGF-2R stimulates cardiomyocyte hypertrophy in the late gestation sheep fetus | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1113/jphysiol.2012.238410 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/349405 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Brooks, D. [0000-0001-9098-3626] | - |
Appears in Collections: | Aurora harvest 7 Medicine publications |
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