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Type: Journal article
Title: Spatial gradients in cell wall composition and transcriptional profiles along elongating maize internodes
Author: Zhang, Q.
Cheetamun, R.
Dhugga, K.
Rafalski, J.
Tingey, S.
Shirley, N.
Taylor, J.
Hayes, K.
Beatty, M.
Bacic, A.
Burton, R.
Fincher, G.
Citation: BMC Plant Biology, 2014; 14(1):27-1-27-19
Publisher: BioMed Central
Issue Date: 2014
ISSN: 1471-2229
Statement of
Qisen Zhang, Roshan Cheetamun, Kanwarpal S Dhugga, J Antoni Rafalski, Scott V Tingey, Neil J Shirley, Jillian Taylor, Kevin Hayes, Mary Beatty, Antony Bacic, Rachel A Burton and Geoffrey B Fincher
Abstract: BACKGROUND: The elongating maize internode represents a useful system for following development of cell walls in vegetative cells in the Poaceae family. Elongating internodes can be divided into four developmental zones, namely the basal intercalary meristem, above which are found the elongation, transition and maturation zones. Cells in the basal meristem and elongation zones contain mainly primary walls, while secondary cell wall deposition accelerates in the transition zone and predominates in the maturation zone. RESULTS: The major wall components cellulose, lignin and glucuronoarabinoxylan (GAX) increased without any abrupt changes across the elongation, transition and maturation zones, although GAX appeared to increase more between the elongation and transition zones. Microarray analyses show that transcript abundance of key glycosyl transferase genes known to be involved in wall synthesis or re-modelling did not match the increases in cellulose, GAX and lignin. Rather, transcript levels of many of these genes were low in the meristematic and elongation zones, quickly increased to maximal levels in the transition zone and lower sections of the maturation zone, and generally decreased in the upper maturation zone sections. Genes with transcript profiles showing this pattern included secondary cell wall CesA genes, GT43 genes, some β-expansins, UDP-Xylose synthase and UDP-Glucose pyrophosphorylase, some xyloglucan endotransglycosylases/hydrolases, genes involved in monolignol biosynthesis, and NAM and MYB transcription factor genes. CONCLUSIONS: The data indicated that the enzymic products of genes involved in cell wall synthesis and modification remain active right along the maturation zone of elongating maize internodes, despite the fact that corresponding transcript levels peak earlier, near or in the transition zone.
Keywords: Cell Wall; Zea mays; Cellulose; Lignin; Xylans; Plant Proteins; Gene Expression Regulation, Plant
Rights: © 2014 Zhang et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
RMID: 0030010219
DOI: 10.1186/1471-2229-14-27
Appears in Collections:Agriculture, Food and Wine publications

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