Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/97453
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Type: Journal article
Title: Lipopeptide biosynthesis in Pseudomonas fluorescens is regulated by the protease complex ClpAP
Author: Song, C.
Sundqvist, G.
Malm, E.
De Bruijn, I.
Kumar, A.
Van De Mortel, J.
Bulone, V.
Raaijmakers, J.
Citation: BMC Microbiology, 2015; 15(1):29-1-29-11
Publisher: BioMed Central
Issue Date: 2015
ISSN: 1471-2180
1471-2180
Statement of
Responsibility: 
Chunxu Song, Gustav Sundqvist, Erik Malm, Irene de Bruijn, Aundy Kumar, Judith van de Mortel, Vincent Bulone, and Jos M Raaijmakers
Abstract: BACKGROUND: Lipopeptides (LP) are structurally diverse compounds with potent surfactant and broad-spectrum antibiotic activities. In Pseudomonas and other bacterial genera, LP biosynthesis is governed by large multimodular nonribosomal peptide synthetases (NRPS). To date, relatively little is known about the regulatory genetic network of LP biosynthesis. RESULTS: This study provides evidence that the chaperone ClpA, together with the serine protease ClpP, regulates the biosynthesis of the LP massetolide in Pseudomonas fluorescens SS101. Whole-genome transcriptome analyses of clpA and clpP mutants showed their involvement in the transcription of the NRPS genes massABC and the transcriptional regulator massAR. In addition, transcription of genes associated with cell wall and membrane biogenesis, energy production and conversion, amino acid transport and metabolism, and pilus assembly were altered by mutations in clpA and clpP. Proteome analysis allowed the identification of additional cellular changes associated to clpA and clpP mutations. The expression of proteins of the citrate cycle and the heat shock proteins DnaK and DnaJ were particularly affected. Combined with previous findings, these results suggest that the ClpAP complex regulates massetolide biosynthesis via the pathway-specific, LuxR-type regulator MassAR, the heat shock proteins DnaK and DnaJ, and proteins of the TCA cycle. CONCLUSIONS: Combining transcriptome and proteome analyses provided new insights into the regulation of LP biosynthesis in P. fluorescens and led to the identification of specific missing links in the regulatory pathways.
Keywords: Pseudomonas fluorescens; Molecular Chaperones; Proteome; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Gene Deletion; Gene Regulatory Networks; Lipopeptides; Serine Proteases
Rights: © 2015 Song et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
RMID: 0030031705
DOI: 10.1186/s12866-015-0367-y
Appears in Collections:Agriculture, Food and Wine publications

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