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Type: Journal article
Title: Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa
Author: Toriyama, K.
Hanaoka, K.
Okada, T.
Watanabe, M.
Citation: FEBS Letters, 1998; 424(3):234-238
Publisher: John Wiley & Son
Issue Date: 1998
ISSN: 0014-5793
Statement of
Kinya Toriyama, Kazunori Hanaoka, Takashi Okada, Masao Watanabe
Abstract: A polyclonal antiserum was raised against the extracellular pollen proteins of Brassica rapa and used for screening the expression cDNA libraries made from immature anthers. We obtained five groups of cDNA clones, including cDNAs similar to PCP1, thioredoxin, and lipid transfer protein (LTP). Recombinant protein of the cDNA clone showing sequence similarity to LTP was demonstrated to bind IgE of a patient allergic to Brassica pollen. The cDNA clone reported here, therefore, represents a novel pollen allergen of Brassica rapa.
Keywords: Anther; Brassica rapa; cDNA cloning; Immunoglobulin E; Lipid transfer protein; Pollen allergen
Rights: © 1998 Federation of European Biochemical Societies
DOI: 10.1016/S0014-5793(98)00174-4
Appears in Collections:Agriculture, Food and Wine publications
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