Bacteroides caecicola sp. nov. and Bacteroides gallinaceum sp. nov., isolated from the caecum of an Indonesian chicken

Abstract

Six strains of anaerobic bacteria, C13EG70^T, C13EG118, C13EG186^T, C13GAMG5, C13GAMG28 and C13GAMG40, were isolated from the caecum of a healthy chicken bred in Bogor, Indonesia. Phylogenetic analysis showed the isolates were separated into two groups. Group I (C13EG70^T and C13EG118) showed nearly identical 16S rRNA gene sequences (99.9 % sequence similarity). Group II (C13EG186^T, C13GAMG5, C13GAMG28 and C13GAMG40) showed nearly identical 16S rRNA gene sequences (>99.4 % sequence similarity). The isolates showed low 16S rRNA gene sequence similarities to recognized species of the genus <i>Bacteroides</i>. High gene sequence similarities were found between type strains (C13EG70^T and C13EG186^T) and <i>Bacteroides salanitronis</i> JCM 13657^T (87.9, 91.5 %, respectively). Physiological, biochemical and genotypic characteristics demonstrated that these strains could be separated from the type strain of <i>B. salanitronis</i>. It is concluded that Group I and Group II represent novel species. Two novel species of the genus <i>Bacteroides</i> are proposed as <i>Bacteroides caecicola</i> sp. nov. (type strain C13EG70^T = LIPI12-4-Ck732^T = JSAT12-4-Ck732^T = InaCC B449^T = NBRC 110958^T) and <i>Bacteroides gallinaceum</i> sp. nov. (type strain C13EG186^T = LIPI12-4-Ck844^T = JSAT12-4-Ck884^T = InaCC B451^T = NBRC 110963^T).