Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/98782
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Type: | Journal article |
Title: | Inflammasome components ASC and AIM2 modulate the acute phase of biomaterial implant-induced foreign body responses |
Author: | Christo, S. Diener, K. Manavis, J. Grimbaldeston, M. Bachhuka, A. Vasilev, K. Hayball, J. |
Citation: | Scientific Reports, 2016; 6(1):20635-1-20635-14 |
Publisher: | Nature Publishing Group |
Issue Date: | 2016 |
ISSN: | 2045-2322 2045-2322 |
Statement of Responsibility: | Susan N. Christo, Kerrilyn R. Diener, Jim Manavis, Michele A. Grimbaldeston, Akash Bachhuka, Krasimir Vasilev & John D. Hayball |
Abstract: | Detailing the inflammatory mechanisms of biomaterial-implant induced foreign body responses (FBR) has implications for revealing targetable pathways that may reduce leukocyte activation and fibrotic encapsulation of the implant. We have adapted a model of poly(methylmethacrylate) (PMMA) bead injection to perform an assessment of the mechanistic role of the ASC-dependent inflammasome in this process. We first demonstrate that ASC(-/-) mice subjected to PMMA bead injections had reduced cell infiltration and altered collagen deposition, suggesting a role for the inflammasome in the FBR. We next investigated the NLRP3 and AIM2 sensors because of their known contributions in recognising damaged and apoptotic cells. We found that NLRP3 was dispensable for the fibrotic encapsulation; however AIM2 expression influenced leukocyte infiltration and controlled collagen deposition, suggesting a previously unexplored link between AIM2 and biomaterial-induced FBR. |
Keywords: | Neutrophils Macrophages Animals Mice, Inbred C57BL Mice, Knockout Mice Foreign Bodies Acute Disease Polymethyl Methacrylate Collagen Type I Collagen Type II Blood Proteins DNA-Binding Proteins Cytokines Biocompatible Materials Microscopy, Fluorescence Enzyme-Linked Immunosorbent Assay Immunophenotyping Peritoneal Lavage Prostheses and Implants Adsorption Apoptosis Regulatory Proteins CARD Signaling Adaptor Proteins Immunity, Innate Inflammasomes NLR Family, Pyrin Domain-Containing 3 Protein |
Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
DOI: | 10.1038/srep20635 |
Published version: | http://dx.doi.org/10.1038/srep20635 |
Appears in Collections: | Aurora harvest 7 Medicine publications |
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hdl_98782.pdf | Published version | 3.42 MB | Adobe PDF | View/Open |
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