Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/98782
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Type: Journal article
Title: Inflammasome components ASC and AIM2 modulate the acute phase of biomaterial implant-induced foreign body responses
Author: Christo, S.
Diener, K.
Manavis, J.
Grimbaldeston, M.
Bachhuka, A.
Vasilev, K.
Hayball, J.
Citation: Scientific Reports, 2016; 6(1):20635-1-20635-14
Publisher: Nature Publishing Group
Issue Date: 2016
ISSN: 2045-2322
2045-2322
Statement of
Responsibility: 
Susan N. Christo, Kerrilyn R. Diener, Jim Manavis, Michele A. Grimbaldeston, Akash Bachhuka, Krasimir Vasilev & John D. Hayball
Abstract: Detailing the inflammatory mechanisms of biomaterial-implant induced foreign body responses (FBR) has implications for revealing targetable pathways that may reduce leukocyte activation and fibrotic encapsulation of the implant. We have adapted a model of poly(methylmethacrylate) (PMMA) bead injection to perform an assessment of the mechanistic role of the ASC-dependent inflammasome in this process. We first demonstrate that ASC(-/-) mice subjected to PMMA bead injections had reduced cell infiltration and altered collagen deposition, suggesting a role for the inflammasome in the FBR. We next investigated the NLRP3 and AIM2 sensors because of their known contributions in recognising damaged and apoptotic cells. We found that NLRP3 was dispensable for the fibrotic encapsulation; however AIM2 expression influenced leukocyte infiltration and controlled collagen deposition, suggesting a previously unexplored link between AIM2 and biomaterial-induced FBR.
Keywords: Neutrophils
Macrophages
Animals
Mice, Inbred C57BL
Mice, Knockout
Mice
Foreign Bodies
Acute Disease
Polymethyl Methacrylate
Collagen Type I
Collagen Type II
Blood Proteins
DNA-Binding Proteins
Cytokines
Biocompatible Materials
Microscopy, Fluorescence
Enzyme-Linked Immunosorbent Assay
Immunophenotyping
Peritoneal Lavage
Prostheses and Implants
Adsorption
Apoptosis Regulatory Proteins
CARD Signaling Adaptor Proteins
Immunity, Innate
Inflammasomes
NLR Family, Pyrin Domain-Containing 3 Protein
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
DOI: 10.1038/srep20635
Appears in Collections:Aurora harvest 7
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