Illuminating RPMI-1640: fluorescence dynamics and optical insights for biological applications
Date
2025
Authors
Rahmatpanahi, A.
Bavali, A.
Farhadi, M.
Bagheri Khoulenjani, S.
Kaviani Samani, S.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Photochemistry and Photobiology A: Chemistry, 2025; 469(116537):1-5
Statement of Responsibility
Conference Name
Abstract
RPMI-1640 is a commonly utilized cell culture medium that serves a variety of purposes in both biological research and clinical investigations. Given that Phenol Red constitutes a significant part of RPMI-1640, it is crucial to evaluate the medium's optical characteristics in fluorescence-based assays to facilitate precise data interpretation in biological studies. This research examines the fluorescence properties of RPMI-1640 within the visible spectral range through the application of laser-induced fluorescence (LIF) spectroscopy and differential spectral analysis (DSA). The absorption spectrum indicates a broad distribution across the visible region, which can be attributed to constituents such as Vitamin B12, Phenol Red, and Riboflavin; however, notable fluorescence emission was observed when excited by wavelengths ranging from 400 to 450 nm. The stability of fluorescence, as measured with a 405 nm laser, exhibited minimal signal degradation over a 30-minute period. Additionally, the angular-dependent fluorescence characteristics and the asymmetries observed in the difference spectra provided evidence for the spatially non-homogeneous inner filter effect (2nd-IFE), wherein the reabsorption of emitted fluorescence modifies both the intensity and spectral profile. DSA further elucidated the 2nd-IFE, identifying isosbestic-like points where fluorescence remains largely unaffected by inner filter effects, thus rendering them appropriate for optical assessments of cellular properties within the culture medium.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2025