On the conservation of function of the Drosophila Fat facets deubiquitinating enzyme and Fam, its mouse homolog

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dc.contributor.authorChen, Xiaoguangen
dc.contributor.authorOverstreet, Erinen
dc.contributor.authorWood, Stephen Andrewen
dc.contributor.authorFischer, J. A.en
dc.contributor.organisationCentre for the Molecular Genetics of Developmenten
dc.contributor.schoolSchool of Molecular and Biomedical Science : Biochemistryen
dc.date.issued2000en
dc.descriptionThe original publication can be found at www.springerlink.comen
dc.description.abstractFat facets is a Drosophila deubiquitinating enzyme required for eye development and early embryogenesis. Genetic evidence suggests that Fat facets deubiquitinates and thereby prevents the proteasomal degradation of specific substrates. The Drosophila Liquid facets protein is implicated as the critical substrate of Fat facets in the eye. A mouse homolog of Fat facets, called Fam, has been identified. The results of biochemical experiments implicate two different proteins, Af-6 and β-catenin, as substrates for Fam. Here, the functional relationship between Fat facets and Fam is explored. We show that Fam can substitute for Fat facets in all of its essential functions in Drosophila. In addition, we tested the hypothesis that Canoe and Armadillo, the Drosophila homologs of Af-6 and β-catenin, respectively, are important substrates for Fat facets in the Drosophila eye. We found no genetic evidence to support a role for either Canoe or Armadillo in the essential Fat facets pathways in Drosophila eye development. The significance of these results is discussed in light of the biochemical experiments that suggest that Af-6 and β-catenin are substrates of Fam.en
dc.identifier.citationDevelopment Genes and Evolution, 2000; 210(12):603-610en
dc.identifier.doi10.1007/s004270000109en
dc.identifier.issn0949-944Xen
dc.identifier.urihttp://hdl.handle.net/2440/28007
dc.language.isoenen
dc.publisherSpringer Verlagen
dc.source.urihttp://www.springerlink.com/content/4c3f81w6e4hl1j6a/en
dc.subjectDeubiquitinating enzyme; β-Cateninen
dc.titleOn the conservation of function of the Drosophila Fat facets deubiquitinating enzyme and Fam, its mouse homologen
dc.typeJournal articleen

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