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A zebrafish melanophore model of amyloidβ toxicity

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dc.contributor.authorNewman, M.
dc.contributor.authorWilson, L.
dc.contributor.authorCamp-Dotlic, E.
dc.contributor.authorVerdile, G.
dc.contributor.authorMartins, R.
dc.contributor.authorLardelli, M.
dc.date.issued2010
dc.description.abstractReliable animal models are required to facilitate the understanding of neurodegenerative pathways in Alzheimer's disease. Animal models can also be employed to search for disease-modifying drugs. The embryos and larvae of zebrafish are particularly advantageous for this purpose. For Alzheimer's disease, drugs that can ameliorate amyloidβ (Aβ) toxicity have therapeutic and/or prophylactic potential. We attempted to generate a zebrafish model of Aβ toxicity that would be viable and fertile but have a highly visible pigmentation phenotype in larvae. The larvae could then be arrayed in microtiter plates to screen compound libraries for drugs acting to reduce Aβ toxicity. We used the promoter of the zebrafish mitfa (nacre) gene to drive expression of the pathological 42 amino acid species of human Aβ, Aβ42, specifically in the highly visible melanophores (melanocytes) of transgenic zebrafish. However, the transgenic fish only showed an aberrant pigment phenotype in adults at the advanced age of 16 months. Nevertheless, our results show that alteration of zebrafish pigment pattern may be useful for analysis of toxic peptide action.
dc.description.statementofresponsibilityMorgan Newman, Lachlan Wilson, Esther Camp, Giuseppe Verdile, Ralph Martins and Michael Lardelli
dc.identifier.citationZebrafish, 2010; 7(2):155-159
dc.identifier.doi10.1089/zeb.2009.0628
dc.identifier.issn1545-8547
dc.identifier.issn1557-8542
dc.identifier.orcidNewman, M. [0000-0002-4930-4529]
dc.identifier.orcidLardelli, M. [0000-0002-4289-444X]
dc.identifier.urihttp://hdl.handle.net/2440/60638
dc.language.isoen
dc.publisherMary Ann Liebert, Inc. Publishers
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/453622
dc.rightsCopyright © 2010, Mary Ann Liebert, Inc. publishers
dc.source.urihttps://doi.org/10.1089/zeb.2009.0628
dc.subjectAnimals
dc.subjectZebrafish
dc.subjectAlzheimer Disease
dc.subjectDisease Models, Animal
dc.subjectZebrafish Proteins
dc.subjectDNA Primers
dc.subjectPigmentation
dc.subjectIn Situ Hybridization
dc.subjectGene Transfer Techniques
dc.subjectPolymerase Chain Reaction
dc.subjectMicrophthalmia-Associated Transcription Factor
dc.subjectDrug Discovery
dc.subjectAmyloid beta-Peptides
dc.titleA zebrafish melanophore model of amyloidβ toxicity
dc.title.alternativeA zebrafish melanophore model of amyloid beta toxicity
dc.typeJournal article
pubs.publication-statusPublished

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