Free oxygen radicals regulate plasma membrane Ca2+- and K+-permeable channels in plant root cells

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dc.contributor.authorDemidchik, V.
dc.contributor.authorShabala, S.
dc.contributor.authorCoutts, K.
dc.contributor.authorTester, M.
dc.contributor.authorDavies, J.
dc.date.issued2003
dc.description.abstractFree oxygen radicals are an irrefutable component of life, underlying important biochemical and physiological phenomena in animals. Here it is shown that free oxygen radicals activate plasma membrane Ca²⁺- and K⁺-permeable conductances in Arabidopsis root cell protoplasts, mediating Ca²⁺ influx and K⁺ efflux, respectively. Free oxygen radicals generate increases in cytosolic Ca²⁺ mediated by a novel population of nonselective cation channels that differ in selectivity and pharmacology from those involved in toxic Na⁺ influx. Analysis of the free oxygen radical-activated K⁺ conductance showed its similarity to the Arabidopsis root K⁺ outward rectifier. Significantly larger channel activation was found in cells responsible for perceiving environmental signals and undergoing elongation. Quenching root free oxygen radicals inhibited root elongation, confirming the role of radical-activated Ca²⁺ influx in cell growth. Net free oxygen radical-stimulated Ca²⁺ influx and K⁺ efflux were observed in root cells of monocots, dicots, C3 and C4 plants, suggesting conserved mechanisms and functions. In conclusion, two functions for free oxygen radical cation channel activation are proposed: initialization/amplification of stress signals and control of cell elongation in root growth.
dc.description.statementofresponsibilityVadim Demidchik, Sergey N. Shabala, Katherine B. Coutts, Mark A. Tester and Julia M. Davies
dc.identifier.citationJournal of Cell Science, 2003; 116(1):81-88
dc.identifier.doi10.1242/jcs.00201
dc.identifier.issn0021-9533
dc.identifier.issn1477-9137
dc.identifier.urihttp://hdl.handle.net/2440/40665
dc.language.isoen
dc.publisherCompany of Biologists Ltd
dc.source.urihttps://doi.org/10.1242/jcs.00201
dc.subjectArabidopsis
dc.subjectcalcium
dc.subjectchannel
dc.subjectfree oxygen radical
dc.subjectplasma membrane
dc.subjectpotassium
dc.titleFree oxygen radicals regulate plasma membrane Ca2+- and K+-permeable channels in plant root cells
dc.typeJournal article
pubs.publication-statusPublished

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