A His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behaviour

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dc.contributor.authorClarkson, M.
dc.contributor.authorSaint, R.
dc.date.issued1999
dc.description.abstractWe have generated Drosophila melanogaster lines carrying a modified genomic fragment which encodes the D. melanogaster variant H2A.F/Z class histone, His2AvD, fused to the green fluorescent protein (GFP) of the jellyfish Aequorea victoria. We show here that the fusion protein consists of functional GFP and functional histone His2AvD. The His2AvD portion of the fusion gene was shown to be functional by rescue of His2AvD mutant lethality. Fluorescence of the fusion protein in vivo was observed in embryonic cleavage stage interphase nuclei and on chromosomes as early as cycle 9, correlating with activation of transcription. Unlike transcription factors, the His2AvDGFP protein remained on transcriptionally inactive chromosomes throughout mitosis. Subsequently, fluorescence was observed in nuclei at all stages of embryonic and larval development and in adult somatic tissues, consistent with the distribution of His2AvD observed by immunohistochemical staining. This functional fusion histone acts as an excellent in vivo marker for chromosomes and chromosome behavior and, given the ability of the fusion gene to prevent null-mutant lethality, without disrupting normal cellular functions. The very high level of conservation of the H2A.F/Z family of variant histones suggests that the equivalent fusion protein construct should function equally well in a wide range of organisms.
dc.identifier.citationDNA and Cell Biology, 1999; 18(6):457-462
dc.identifier.doi10.1089/104454999315178
dc.identifier.issn1044-5498
dc.identifier.issn1557-7430
dc.identifier.urihttp://hdl.handle.net/2440/11282
dc.language.isoen
dc.publisherMARY ANN LIEBERT INC PUBL
dc.source.urihttps://doi.org/10.1089/104454999315178
dc.subjectChromosomes
dc.subjectAnimals
dc.subjectDrosophila melanogaster
dc.subjectScyphozoa
dc.subjectLuminescent Proteins
dc.subjectGreen Fluorescent Proteins
dc.subjectHistones
dc.subjectRecombinant Fusion Proteins
dc.subjectGenetic Markers
dc.subjectMicroscopy, Fluorescence
dc.subjectTranscription, Genetic
dc.subjectMutation
dc.subjectAlleles
dc.subjectGenes, Lethal
dc.subjectGenetic Vectors
dc.subjectNuclear Localization Signals
dc.titleA His2AvDGFP fusion gene complements a lethal His2AvD mutant allele and provides an in vivo marker for Drosophila chromosome behaviour
dc.typeJournal article
pubs.publication-statusPublished

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