Caspase-2 protocols
Date
2014
Authors
Dorstyn, L.
Kumar, S.
Editors
Bozhkov, P.V.
Salvesen, G.
Salvesen, G.
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Journal article
Citation
Methods in Molecular Biology, 2014; 1133:71-87
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Abstract
Caspase-2 has been shown to function in apoptosis and in some non-apoptotic pathways, including tumor suppression and aging. Caspase-2 has some unique features and is the only caspase that constitutively localizes to the nucleus, although its nuclear function remains unknown. During apoptosis signaling, caspase-2 rapidly homodimerizes, which leads to its activation and proteolytic processing. The activation of caspase-2 can be measured by assessing its dimerization and/or cleavage of the caspase-2 zymogen and its substrates. This chapter outlines commonly used methods to purify recombinant caspase-2 and assess its activity and function in vitro and in cultured cells or tissue extracts.
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Copyright 2014 Springer Science+Business Media New York