Hepatitis B virus and hepatitis C virus interaction in Huh-7 cells

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dc.contributor.authorEyre, N.
dc.contributor.authorPhillips, R.
dc.contributor.authorBowden, S.
dc.contributor.authorYip, L.
dc.contributor.authorDewar, B.
dc.contributor.authorLocarnini, S.
dc.contributor.authorBeard, M.
dc.date.issued2009
dc.description2009 Published by Elsevier B.V. on behalf of the European Association for the study of the Liver. All rights reserved.
dc.description.abstract<h4>Background/aims</h4>Co-infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) increases the risk of development and the severity of chronic liver disease. Although dominant and suppressive effects of each virus over the other have been reported in vivo, in vitro studies of HBV/HCV co-infection have been limited to analysis of the effects of over-expression of HCV proteins on HBV replication.<h4>Methods</h4>We have re-examined HBV/HCV interactions in Huh-7 cells following co-infection with cell culture-propagated HCV (HCVcc; genotype 2a) and a recombinant adenovirus vector capable of delivering a replication-competent HBV genome (AdHBV; genotype A).<h4>Results</h4>While intracellular HCV RNA levels were significantly increased when cells were pre-infected with AdHBV, HCV replication and virion secretion were not altered by simultaneous infection with AdHBV or AdHBV superinfection of HCV-infected cells. Likewise intracellular and secreted HBV DNA levels and HBV promoter activities were either unchanged or modestly increased by HCVcc infection. Despite this, HCV E2 and HBsAg proteins colocalized extensively in co-infected cells suggesting shared stages in viral egress.<h4>Conclusions</h4>These studies indicate that there is little direct interaction of HBV and HCV in co-infected hepatocytes and imply that indirect effects of host-viral interactions dictate viral dominance in HBV/HCV co-infected individuals.
dc.description.statementofresponsibilityNicholas S. Eyre, Renee J. Phillips, Scott Bowden, Evelyn Yip, Ben Dewar, Stephen A. Locarnini and Michael R. Beard
dc.identifier.citationJournal of Hepatology, 2009; 51(3):446-457
dc.identifier.doi10.1016/j.jhep.2009.04.025
dc.identifier.issn0168-8278
dc.identifier.issn1600-0641
dc.identifier.orcidEyre, N. [0000-0002-5424-7573]
dc.identifier.orcidPhillips, R. [0000-0001-8340-9319]
dc.identifier.orcidBeard, M. [0000-0002-4106-1016]
dc.identifier.urihttp://hdl.handle.net/2440/54737
dc.language.isoen
dc.publisherElsevier BV
dc.source.urihttps://doi.org/10.1016/j.jhep.2009.04.025
dc.subjectHBV
dc.subjectHCV
dc.subjectCo-infection
dc.subjectSuperinfection
dc.subjectHepatocytes
dc.subjectPathogenesis
dc.titleHepatitis B virus and hepatitis C virus interaction in Huh-7 cells
dc.typeJournal article
pubs.publication-statusPublished

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