The effects of stubble retention and nitrogen application on soil microbial community structure and functional gene abundance under irrigated maize
Date
2007
Authors
Wakelin, S.
Colloff, M.
Harvey, P.
Marschner, P.
Gregg, A.
Rogers, S.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
FEMS Microbiology Ecology, 2007; 59(3):661-670
Statement of Responsibility
Steven A. Wakelin, Matt J. Colloff, Paul R. Harvey, Petra Marschner, Adrienne L. Gregg & Stephen L. Rogers
Conference Name
Abstract
The effects of agronomic management practices on the soil microbial community were investigated in a maize production system in New South Wales, Australia. The site has been intensively studied to measure the impact of stubble management and N-fertilizer application on greenhouse gas emissions (CO₂ and N₂O), N-cycling, pathology, soil structure and yield. As all of these endpoints can be regulated by microbial processes, the microbiology of the system was examined. Soil samples were taken after a winter fallow period and the diversity of the bacterial and fungal communities was measured using PCR-denaturing gradient gel electrophoresis. Stubble and N shifted the structure of bacterial and fungal communities with the primary driver being stubble addition on the fungal community structure (P<0.05 for all effects). Changes in C, N (total and NO3), K and Na, were correlated (P<0.05) with variation in the microbial community structure. Quantitative PCR showed that nifH (nitrogen fixation) and napA (denitrification) gene abundance increased upon stubble retention, whereas amoA gene numbers were increased by N addition. These results showed that the management of both stubble and N have significant and long-term impacts on the size and structure of the soil microbial community at phylogenetic and functional levels.
School/Discipline
Dissertation Note
Provenance
Description
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