Control of gene expression in the P2-related template coliphages
| dc.contributor.author | Kalionis, B. | |
| dc.contributor.author | Dodd, I.B. | |
| dc.contributor.author | Egan, J.B. | |
| dc.date.issued | 1986 | |
| dc.description.abstract | The PstI fragment (65.5% to 77.4%) of coliphage 186, known genetically to encode the major control genes, has been sequenced, and an analysis performed to assess coding capacity, transcription-translation signals, and to identify any other significant features. Our analysis indicates that the region encodes: (1) seven genes, including the int and cI genes, which overlap, the late control gene B, and two genes, named CP75 and CP76, encoding potential DNA-binding proteins; (2) a promoter pB and terminator tB for the rightward transcription of the B gene, and we predict the existence of this transcript in a lysogen; (3) a promoter pL and terminator tL for leftward transcription that encodes the int and cI genes, and represents the presumed lysogenic transcript; (4) a promoter pR for rightward transcription to give the presumed (early) lytic transcript that is overlapping and convergent with the lysogenic transcript; and finally (5), a potential operator site for repressor binding in the region of the pR promoter. Preliminary evidence is presented to support this analysis. | |
| dc.description.statementofresponsibility | Bill Kalionis, Ian B. Dodd, J.Barry Egan | |
| dc.identifier.citation | Journal of Molecular Biology, 1986; 191(2):199-209 | |
| dc.identifier.doi | 10.1016/0022-2836(86)90257-3 | |
| dc.identifier.issn | 0022-2836 | |
| dc.identifier.issn | 1089-8638 | |
| dc.identifier.orcid | Dodd, I.B. [0000-0003-2969-6841] | |
| dc.identifier.uri | http://hdl.handle.net/2440/87412 | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.rights | © 1986 Academic Press, Inc. | |
| dc.source.uri | https://doi.org/10.1016/0022-2836(86)90257-3 | |
| dc.subject | Ribosomes | |
| dc.subject | Coliphages | |
| dc.subject | DNA, Viral | |
| dc.subject | Transcription, Genetic | |
| dc.subject | Gene Expression Regulation | |
| dc.subject | Binding Sites | |
| dc.subject | Base Sequence | |
| dc.subject | Repetitive Sequences, Nucleic Acid | |
| dc.subject | Promoter Regions, Genetic | |
| dc.subject | Terminator Regions, Genetic | |
| dc.title | Control of gene expression in the P2-related template coliphages | |
| dc.type | Journal article | |
| pubs.publication-status | Published |