NIPAM-based microgel microenvironment regulates the therapeutic function of cardiac stromal cells

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dc.contributor.authorCui, X.
dc.contributor.authorTang, J.
dc.contributor.authorHartanto, Y.
dc.contributor.authorZhang, J.
dc.contributor.authorBi, J.
dc.contributor.authorDai, S.
dc.contributor.authorQiao, S.
dc.contributor.authorCheng, K.
dc.contributor.authorZhang, H.
dc.date.issued2018
dc.description.abstractTo tune the chemical, physical, and mechanical microenvironment for cardiac stromal cells to treat acute myocardial infarction (MI), we prepared a series of thermally responsive microgels with different surface charges (positive, negative, and neutral) and different degrees of hydrophilicity, as well as functional groups (carboxyl, hydroxyl, amino, and methyl). These microgels were used as injectable hydrogels to create an optimized microenvironment for cardiac stromal cells (CSCs). Our results indicated that a hydrophilic and negatively charged microenvironment created from poly( N-isopropylacrylamide- co-itaconic acid) was favorable for maintaining high viability of CSCs, promoting CSC proliferation and facilitating the formation of CSC spheroids. A large number of growth factors, such as vascular endothelial growth factor (VEGF), insulin-like growth factor I (IGF-1), and stromal-derived factor-1 (SDF-1) were released from the spheroids, promoting neonatal rat cardiomyocyte activation and survival. After injecting the poly( N-isopropylacrylamide- co-itaconic acid) microgel into mice, we examined their acute inflammation and T-cell immune reactions. The microgel itself did not elicit obvious immune response. We then injected the same microgel-encapsulated with CSCs into MI mice. The result revealed the treatment-promoted MI heart repair through angiogenesis and inhibition of apoptosis with an improved cell retention rate. This study will open a door for tailoring poly( N-isopropylacrylamide)-based microgel as a delivery vehicle for CSC therapy.
dc.description.statementofresponsibilityXiaolin Cui, Junnan Tang, Yusak Hartanto, Jiabin Zhang, Jingxiu Bi, Sheng Dai, Shi Zhang Qiao, Ke Cheng, and Hu Zhang
dc.identifier.citationACS applied materials & interfaces, 2018; 10(44):37783-37796
dc.identifier.doi10.1021/acsami.8b09757
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.orcidBi, J. [0000-0001-7056-8572]
dc.identifier.orcidQiao, S. [0000-0002-1220-1761] [0000-0002-4568-8422]
dc.identifier.orcidZhang, H. [0000-0003-4178-6401]
dc.identifier.urihttp://hdl.handle.net/2440/117735
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/DP160104632
dc.rights© 2018 American Chemical Society
dc.source.urihttps://doi.org/10.1021/acsami.8b09757
dc.subjectInjectable hydrogels; NIPAM microgel; thermo-responsive microgel; cardiac stromal cells; multicellular spheroids; heart repair
dc.titleNIPAM-based microgel microenvironment regulates the therapeutic function of cardiac stromal cells
dc.typeJournal article
pubs.publication-statusPublished

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