ElectroSens platform with a polyelectrolyte-based carbon fiber sensor for point-of-care analysis of Zn in blood and urine
Date
2020
Authors
Nikolaev, K.G.
Kalmykov, E.V.
Shavronskaya, D.O.
Nikitina, A.A.
Stekolshchikova, A.A.
Kosareva, E.A.
Zenkin, A.A.
Pantiukhin, I.S.
Orlova, O.Y.
Skalny, A.V.
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Journal article
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ACS Omega, 2020; 5(30):18987-18994
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Abstract
In this paper, we describe an electrochemical sensing platform - ElectroSens - for the detection of Zn based on self-assembled polyelectrolyte multilayers on the carbon fiber (CF) electrode surface. The CF-extended surface facilitates the usage of a small volume electrochemical cell (1 mL) without stirring. This approach allows making a low-cost three-electrode platform. Working electrode modification with layer-by-layer assembly of polyethyleneimine (PEI), poly(sodium 4-styrenesulfonate) (PSS), and mercury nitrate layers eliminates solution toxicity andprovides stable stripping voltammetry measurements. The stable, robust, sustainable, and even reusable Ag/AgCl reference electrode consists of adsorbed 32 PEI-KCl/PSSKCl bilayers on the CF/silver paste separated from the outer solution by a polyvinylchloride membrane. The polyelectrolyte-based sensor interface prevents adsorption of protein molecules from biological liquids on the CF surface that leads to a sensitivity increase of up to 2.2 μA/M for Zn2+ detection and provides a low limit of detection of 4.6 × 10−8 M. The linear range for Zn detection is 1 × 10−7 to 1 × 10−5 M. A portable potentiostat connected via wireless to a smartphone with an android-based software is also provided. The ElectroSens demonstrates reproducibility and repeatability of data for the detection of Zn in blood and urine without the digestion step.
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Data source: Supporting information, https://doi.org/10.1021/acsomega.0c02279
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Copyright 2020 American Chemical Society. This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes. (https://pubs.acs.org/page/policy/authorchoice_termsofuse.html)