Pleiotropic mutants of Aspergillus nidulans altered in carbon metabolism

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dc.contributor.authorHynes, M.J.
dc.contributor.authorKelly, J.M.
dc.date.issued1977
dc.description.abstractMutants altered in carbon catabolite regulation have been isolated by selecting for mutants of theareA217 strain capable of using acetamide as the sole nitrogen source in the presence of sucrose. In addition tocreA mutants described previously by Arst and Cove, strains with mutations in two new genes,creB andcreC, have been found. ThecreB andcreC mutants grow poorly on some sole carbon sources and have low levels of some enzymes of carbon catabolism e.g. β-galactosidase and D-quinate dehydrogenase. ThecreB andcreC mutants are hypersitive to fluoroacetate, fluoroacetamide and allyl alcohol in the presence of glucose or sucrose but not glycerol; and the enzymes, acetamidase, and alcohol dehydrogenase, are less sensitive to carbon catabolite repression than the wild-type strain. Extracellular protease and α-glucosidase enzyme activities are elevated increB andcreC mutants, while L-proline and L-glutamate uptake capacities are lower in both the presence and absence of glucose. Interactions betweencreA, B and C mutations have been investigated in double mutants, and the dominance properties ofcreB andcreC mutants determined. The results indicate that thecreB andcreC genes may have a regulatory role in the control of carbon catabolism.
dc.description.statementofresponsibilityM.J. Hynes and Joan M. Kelly
dc.identifier.citationMolecular Genetics and Genomics: an international journal, 1977; 150(2):193-204
dc.identifier.doi10.1007/BF00695399
dc.identifier.issn0026-8925
dc.identifier.issn1432-1874
dc.identifier.urihttp://hdl.handle.net/2440/85727
dc.language.isoen
dc.publisherSpringer-Verlag
dc.rights© by Springer-Verlag 1977
dc.source.urihttps://doi.org/10.1007/bf00695399
dc.subjectAspergillus nidulans
dc.subjectCarbon
dc.subjectAcetamides
dc.subjectAmidohydrolases
dc.subjectGalactosidases
dc.subjectGlucosidases
dc.subjectAlcohol Oxidoreductases
dc.subjectGlutamate Dehydrogenase
dc.subjectSucrose
dc.subjectMutation
dc.subjectGenes, Regulator
dc.titlePleiotropic mutants of Aspergillus nidulans altered in carbon metabolism
dc.typeJournal article
pubs.publication-statusPublished

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