A family of RS domain proteins with novel subcellular localization and trafficking

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dc.contributor.authorKavanagh, S.
dc.contributor.authorSchulz, T.
dc.contributor.authorDavey, P.
dc.contributor.authorClaudianos, C.
dc.contributor.authorRussell, C.
dc.contributor.authorRathjen, P.
dc.contributor.organisationCentre for the Molecular Genetics of Development
dc.date.issued2005
dc.description.abstractWe report the sequence, conservation and cell biology of a novel protein, Psc1, which is expressed and regulated within the embryonic pluripotent cell population of the mouse. The Psc1 sequence includes an RS domain and an RNA recognition motif (RRM), and a sequential arrangement of protein motifs that has not been demonstrated for other RS domain proteins. This arrangement was conserved in a second mouse protein (BAC34721. The identification of Psc1 and BAC34721homologues in vertebrates and related proteins, more widely throughout evolution, defines a new family of RS domain proteins termed acidic rich RS (ARRS) domain proteins. Psc1 incorporated into the nuclear speckles, but demonstrated novel aspects of subcellular distribution including localization to speckles proximal to the nuclear periphery and localization to punctate structures in the cytoplasm termed cytospeckles. Integration of Psc1 into cytospeckles was dependent on the RRM. Cytospeckles were dynamic within the cytoplasm and appeared to traffic into the nucleus. These observations suggest a novel role in RNA metabolism for ARRS proteins.
dc.identifier.citationNucleic Acids Research, 2005; 33(4):1309-1322
dc.identifier.doi10.1093/nar/gki269
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.urihttp://hdl.handle.net/2440/27607
dc.language.isoen
dc.publisherOxford Univ Press
dc.source.urihttp://nar.oxfordjournals.org/cgi/content/abstract/33/4/1309
dc.subjectCOS Cells
dc.subjectCell Nucleus
dc.subjectCell Nucleus Structures
dc.subjectCytoplasm
dc.subjectCytoplasmic Structures
dc.subjectMicrotubules
dc.subjectAnimals
dc.subjectMice
dc.subjectProteins
dc.subjectRNA-Binding Proteins
dc.subjectNuclear Proteins
dc.subjectDNA, Complementary
dc.subjectEvolution, Molecular
dc.subjectBinding Sites
dc.subjectAmino Acid Sequence
dc.subjectConserved Sequence
dc.subjectProtein Structure, Tertiary
dc.subjectActive Transport, Cell Nucleus
dc.subjectMolecular Sequence Data
dc.subjectChlorocebus aethiops
dc.titleA family of RS domain proteins with novel subcellular localization and trafficking
dc.typeJournal article
pubs.publication-statusPublished

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