The central role of arginine in Haemophilus influenzae survival in a polymicrobial environment with Streptococcus pneumoniae and Moraxella catarrhalis

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dc.contributor.authorTikhomirova, A.
dc.contributor.authorZilm, P.S.
dc.contributor.authorTrappetti, C.
dc.contributor.authorPaton, J.C.
dc.contributor.authorKidd, S.P.
dc.contributor.editorvan Veen, H.W.
dc.date.issued2022
dc.description.abstractHaemophilus influenzae, Streptococcus pneumoniae and Moraxella catarrhalis are bacterial species which frequently co-colonise the nasopharynx, but can also transit to the middle ear to cause otitis media. Chronic otitis media is often associated with a polymicrobial infection by these bacteria. However, despite being present in polymicrobial infections, the molecular interactions between these bacterial species remain poorly understood. We have previously reported competitive interactions driven by pH and growth phase between H. influenzae and S. pneumoniae. In this study, we have revealed competitive interactions between the three otopathogens, which resulted in reduction of H. influenzae viability in co-culture with S. pneumoniae and in triple-species culture. Transcriptomic analysis by mRNA sequencing identified a central role of arginine in mediating these interactions. Arginine supplementation was able to increase H. influenzae survival in a dual-species environment with S. pneumoniae, and in a triple-species environment. Arginine was used by H. influenzae for ATP production, and levels of ATP generated in dual- and triple-species co-culture at early stages of growth were significantly higher than the combined ATP levels of single-species cultures. These results indicate a central role for arginine-mediated ATP production by H. influenzae in the polymicrobial community.
dc.description.statementofresponsibilityAlexandra TikhomirovaID, Peter S. Zilm, Claudia Trappetti, James C. Paton, Stephen P. Kidd
dc.identifier.citationPLoS ONE, 2022; 17(7):1-16
dc.identifier.doi10.1371/journal.pone.0271912
dc.identifier.issn1932-6203
dc.identifier.issn1932-6203
dc.identifier.orcidTikhomirova, A. [0000-0001-9920-0245]
dc.identifier.orcidZilm, P.S. [0000-0001-7554-9717]
dc.identifier.orcidTrappetti, C. [0000-0001-8272-0068]
dc.identifier.orcidPaton, J.C. [0000-0001-9807-5278]
dc.identifier.orcidKidd, S.P. [0000-0002-2118-1651]
dc.identifier.urihttps://hdl.handle.net/2440/136995
dc.language.isoen
dc.publisherPublic Library of Science (PLoS)
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1174876
dc.rights© 2022 Tikhomirova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1371/journal.pone.0271912
dc.subjectHumans
dc.subjectHaemophilus influenzae
dc.subjectStreptococcus pneumoniae
dc.subjectOtitis Media
dc.subjectArginine
dc.subjectAdenosine Triphosphate
dc.subjectCoinfection
dc.subjectMoraxella catarrhalis
dc.subject.meshHumans
dc.subject.meshHaemophilus influenzae
dc.subject.meshStreptococcus pneumoniae
dc.subject.meshOtitis Media
dc.subject.meshArginine
dc.subject.meshAdenosine Triphosphate
dc.subject.meshCoinfection
dc.subject.meshMoraxella catarrhalis
dc.titleThe central role of arginine in Haemophilus influenzae survival in a polymicrobial environment with Streptococcus pneumoniae and Moraxella catarrhalis
dc.typeJournal article
pubs.publication-statusPublished

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